Pre-weaning performance and health of pigs born to cloned (fetal cell derived) swine versus non-cloned swine
Pre-weaning performance and health of pigs born to cloned (fetal cell derived) swine versus non-cloned swine
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DOI：
10.1016/j.theriogenology.2003.08.003
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发表时间：
2004-07-01
期刊：
影响因子：
2.8
通讯作者：
Wiseman, B
中科院分区：
文献类型：
--
作者：
Martin, M;Adams, C;Wiseman, B
本研究的目的是比较克隆亲本和非克隆亲本衍生猪的早期断奶性能。用5头克隆母猪和1头克隆公猪生产5窝猪。五只克隆雌性中的一只和克隆的公猪来自两个未经基因处理的胎儿成纤维细胞系。其余的雌性克隆来自胎儿成纤维细胞系，在该细胞系中发生了α-1，3-半乳糖基转移酶基因靶向构建体的随机插入。胎儿细胞系具有相似的遗传背景，来自三个不同胎次的三个不同胎儿。两头非克隆公猪与五头非克隆母猪交配也产下了五窝猪。克隆亲本与非克隆亲本母猪的平均孕长、平均产仔数、平均初生重和断奶重相似。与非克隆亲本相比，克隆亲本出生的生猪和断奶后存活的猪的比例也相似。总之，来自胎儿成纤维细胞系的克隆猪与非克隆猪交配产生的一窝猪在出生数量、仔猪出生体重和围产期和断奶前死亡率方面与非克隆猪相似。(C)2003 Elsevier Inc.保留所有权利。
The objective of this study was to compare the pre-weaning performance of pigs derived from cloned versus non-cloned parents. Five cloned gilts and one cloned boar were used to produce five litters of pigs. One of five cloned females and the cloned boar were derived from two genetically unmanipulated fetal fibroblast cell lines. The remaining female clones were derived from a fetal fibroblast cell line in which random insertion of a alpha-1,3-galactosyltransferase gene targeting construct had occurred. Fetal cell lines had similar genetic backgrounds and were derived from three different fetuses in three different litters. Five litters of pigs were also generated from matings between two non-cloned boars and five non-cloned gilts. The mean gestation length, mean litter size, mean birth and weaning weights for male and female pigs were similar for litters derived from cloned parents versus non-cloned parents. The proportions of pigs born live and pigs that survived to weaning were also similar for pigs born to cloned as compared to non-cloned parents. In summary, matings between cloned swine derived from fetal fibroblast cell lines yielded litters of pigs that were similar in the number born, piglet birth weight and perinatal and pre-weaning mortality to litters produced by non-cloned swine. (C) 2003 Elsevier Inc. All rights reserved.