Bio-inspired hard-to-soft interface for implant integration to bone.
Bio-inspired hard-to-soft interface for implant integration to bone.
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DOI：
10.1016/j.nano.2014.10.003
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Tamerler C
中科院分区：
文献类型：
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作者：
Zhou Y;Snead ML;Tamerler C
在宿主与生物材料界面上实现完整的功能集成一直是实现与生物材料性能相似的工程植入物的关键障碍。基于分子识别的自组装，加上生化信号，可能导致可控制和可预测的细胞分化在植入物界面。在这里，我们设计了一个基于嵌合肽的仿生界面。与生物材料界面的结合是通过钛/钛合金植入物表面特异性的分子识别结构域和通过激活骨形成的Wnt信号通路引导干细胞分化的生化信号来实现的。在宿主细胞生长和决定的关键时期，生物活性植入物界面向小鼠和人类干细胞发出信号，使其沿成骨谱系分化。wnt诱导的细胞在其生成的细胞外基质中显示出增强的矿物质沉积，并且增强了与成骨一致的基因表达谱，从而提供了促进骨再生的骨与植入物界面。
Accomplishing full, functional integration at the host-to-biomaterial interface has been a critical roadblock in engineering implants with performance similar to biological materials. Molecular recognition-based self-assembly, coupled with biochemical signaling, may lead to controllable and predictable cellular differentiation at the implant interface. Here, we engineer a bio-inspired interface built upon a chimeric peptide. Binding to the biomaterial interface is achieved using a molecular recognition domain specific for the titanium/titanium alloy implant surface and a biochemical signal guiding stem cells to differentiate by activating the Wnt signaling pathway for bone formation. During a critical period of host cell growth and determination, the bioactive implant interface signals mouse, as well as human, stem cells to differentiate along osteogenic lineages. The Wnt-induced cells show enhanced mineral deposition in an extracellular matrix of their creation and an enhanced gene expression profile consistent with osteogenesis, thereby providing a bone-to-implant interface that promotes bone regeneration.