Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses
Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses
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针对乙型肝炎和人类免疫缺陷病毒的新型二价植物口服疫苗的免疫原性
DOI：
10.1007/s10529-006-9028-4
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发表时间：
2006
影响因子：
2.7
通讯作者：
R. Hammond
中科院分区：
文献类型：
--
作者：
S. Shchelkunov;R. K. Salyaev;S. G. Pozdnyakov;N. Rekoslavskaya;A. E. Nesterov;T. S. Ryzhova;V. M. Sumtsova;N. V. Pakova;U. O. Mishutina;T. V. Kopytina;R. Hammond
合成了编码人类免疫缺陷病毒（HIV-1）免疫原性ENV和GAG表位以及乙型肝炎病毒（HBV）表面蛋白抗原（HBsAg）的嵌合基因TBI-HBS在番茄中表达。将含有TBI-HBS抗原的番茄果实喂给实验小鼠，在喂食后的第14天和第28天，实验动物的血清和粪便中存在高水平的HIV和hbv特异性抗体。腹腔注射一种DNA疫苗，指导合成相同的TBI-HBsAg抗原，增强了血清中对HIV的抗体反应；然而，它对产生高水平的HBV抗体没有影响。
A synthetic chimeric gene, TBI-HBS, encoding the immunogenic ENV and GAG epitopes of human immunodeficiency virus (HIV-1) and the surface protein antigen (HBsAg) of hepatitis B virus (HBV), was expressed in tomato plants. Tomato fruits containing the TBI-HBS antigen were fed to experimental mice and, on days 14 and 28 post-feeding, high levels of HIV- and HBV-specific antibodies were present in the serum and feces of the test animals. Intraperitoneal injection of a DNA vaccine directing synthesis of the same TBI-HBsAg antigen boosted the antibody response to HIV in the blood serum; however, it had no effect on the high level of antibodies produced to HBV.
DOI：
10.1073/pnas.0409899102
发表时间：
2005-03-01
影响因子：
11.1
作者：
Thanavala, Y;Mahoney, M;Mason, HS
通讯作者：
Mason, HS