Cloning of the pathogenicity-related gene FPD1 in Fusarium oxysporum f. sp. lycopersici.
Cloning of the pathogenicity-related gene FPD1 in Fusarium oxysporum f. sp. lycopersici.
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DOI：
10.1007/s10327-003-0089-0
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发表时间：
2004-02-01
影响因子：
1.2
通讯作者：
Arie, Tsutomu
中科院分区：
文献类型：
--
作者：
Kawabe, Masato;Mizutani, Kohei;Arie, Tsutomu
本研究选择了一个致病力降低的尖孢镰刀菌突变体。番茄枯萎病病原体番茄斑点落叶病菌（sp.lycopersici）的转化子。预测突变体中用质粒标记的基因编码321个氨基酸的蛋白质，并将其命名为FPD 1。同源性搜索显示其与非洲爪蟾的氯离子传导调节蛋白部分相似，表明FPD 1是一个跨膜蛋白。虽然FPD1的功能尚未明确，但它确实参与了F. oxysporum F. FPD1缺陷突变体对番茄的致病性降低。
We selected a reduced-pathogenicity mutant of Fusarium oxysporum f. sp. lycopersici, a tomato wilt pathogen, from the transformants generated by restriction enzyme-mediated integration (REMI) transformation. The gene tagged with the plasmid in the mutant was predicted to encode a protein of 321 amino acids and was designated FPD1. Homology search showed its partial similarity to a chloride conductance regulatory protein of Xenopus, suggesting that FPD1 is a transmembrane protein. Although the function of FPD1 has not been identified, it does participate in the pathogenicity of F. oxysporum f. sp. lycopersici because FPD1-deficient mutants reproduced the reduced pathogenicity on tomato.