Interaction of the Monoclonal Antibodies αlR-1 and αIR-3 with Insulin and Somatomedin-C Receptors
Interaction of the Monoclonal Antibodies αlR-1 and αIR-3 with Insulin and Somatomedin-C Receptors
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单克隆抗体 αlR-1 和 αIR-3 与胰岛素和生长调节素-C 受体的相互作用
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
J. J. Wyk
中科院分区：
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作者：
S. Jacobs;S. Cook;M. Svoboda;J. J. Wyk
αIR-3是一种与生长调节素-C受体相互作用的单克隆抗体，可抑制生长调节素- C与人胎盘膜和完整IM-9细胞的结合，但不抑制胰岛素与人胎盘膜和完整IM-9细胞的结合。αIR-1是一种与胰岛素受体相互作用的单克隆抗体，不抑制任何一种激素的结合。αIR-3对生长调节素-C结合的抑制作用主要是由于其亲和力降低。125 I标记的αIR-3特异性结合胎盘膜和完整的IM-9细胞，并被浓度低于抑制生长调节素-C结合所需浓度的未标记αIR-3抑制。[125 I]αIR-3结合也受到生长调节素-C和胰岛素的抑制，但仅在极高浓度下。A410是一种与胰岛素和生长调节素- C受体均反应的兔抗血清，也可抑制标记的αIR-3结合。αIR-I没有。这些结果有助于确定这些抗体相互作用的表位。（内分泌学118：223-226，1986）
αIR-3, a monoclonal antibody that interacts with the somatomedin-C receptor, inhibited the binding of somatomedin- C, but not of insulin, to human placental membranes and intact IM-9 cells. αIR-1, a monoclonal antibody that interacts with the insulin receptor, did not inhibit the binding of either hormone. Inhibition of somatomedin-C binding by αIR-3 was mainly due to a decrease in its affinity. 125I-Labeled αIR-3 bound specifically to placental membranes and intact IM-9 cells and was inhibited by concentrations of unlabeled αIR-3 that were lower than those required to inhibit somatomedin-C binding. [125I]αIR-3 binding was also inhibited by somatomedin-C and insulin, but only at very high concentrations. A410, a rabbit antiserum that reacts with both receptors for insulin and somatomedin- C, also inhibited labeled αIR-3 binding. αIR-I did not. These results help to define the epitopes with which these antibodies interact. (Endocrinology 118: 223-226, 1986)