Molecular characterization of gentamicin-resistant enterococci in the United States:: Evidence of spread from animals to humans through food
Molecular characterization of gentamicin-resistant enterococci in the United States:: Evidence of spread from animals to humans through food
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DOI：
10.1128/jcm.41.3.1109-1113.2003
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发表时间：
2003-03-01
影响因子：
9.4
通讯作者：
Zervos, MJ
中科院分区：
文献类型：
--
作者：
Donabedian, SM;Thal, LA;Zervos, MJ
我们评估了360种庆大霉素MIC大于或等于128 μ g/ml的肠球菌耐药的分子机制。通过PCR在来自动物、食物和人类的分离物中鉴定aac（6 '）-Ie-aph（2”）-Ia、aph（2”）-Ic和aph（2”）-Id基因。在所有分离株中均未发现aph（2”）-Ib基因。来自动物的两株粪肠球菌分离株（MIC> 1，024 μ g/ml）未能产生任何测试基因的PCR产物，可能含有新的未鉴定的氨基糖苷类耐药基因。脉冲场凝胶电泳（PFGE）分析表明，菌株的多样性。1例人和18例猪大肠杆菌（E.来自密歇根州的具有aac（6 '）-Ie-aph（2”）-Ia基因的粪肠球菌分离株具有相关的PFGE图谱，2株粪肠球菌具有相关的PFGE图谱。来自俄勒冈州的粪肠球菌分离株（1个人和1个杂货店鸡分离株）具有不可区分的PFGE模式。我们发现，当庆大霉素耐药基因存在于来自动物的耐药肠球菌中时，该基因也存在于从相同动物物种的食品中分离的肠球菌中。虽然这些数据表明庆大霉素耐药肠球菌之间存在很大的多样性，但这些数据也表明从不同地理区域的人类、零售食品和农场动物中分离的肠球菌之间庆大霉素耐药性的相似性，并提供了庆大霉素耐药肠球菌通过食品供应从动物传播到人类的证据。
We evaluated the molecular mechanism for resistance of 360 enterococci for which the gentamicin MICs were greater than or equal to128 mug/ml. The aac(6')-Ie-aph(2")-Ia, aph(2")-Ic, and aph(2")-Id genes were identified by PCR in isolates from animals, food, and humans. The aph(2")-Ib gene was not identified in any of the isolates. Two Enterococcus faecalis isolates (MICs > 1,024 mug/ml) from animals failed to generate a PCR product for any of the genes tested and likely contain a new unidentified aminoglycoside resistance gene. Pulsed-field gel electrophoresis (PFGE) analysis showed a diversity of strains. However, 1 human and 18 pork E. faecalis isolates from Michigan with the aac(6')-Ie-aph(2")-Ia gene had related PFGE patterns and 2 E. faecalis isolates from Oregon (1 human and 1 grocery store chicken isolate) had indistinguishable PFGE patterns. We found that when a gentamicin-resistant gene was present in resistant enterococci from animals, that gene was also present in enterococci isolated from food products of the same animal species. Although these data indicate much diversity among gentamicin-resistant enterococci, the data also suggest similarities in gentamicin resistance among enterococci isolated from humans, retail food, and farm animals from geographically diverse areas and provide evidence of the spread of gentamicin-resistant enterococci from animals to humans through the food supply.