Phylogenetic analysis of hepatitis E virus isolates from India (1976-1993)
Phylogenetic analysis of hepatitis E virus isolates from India (1976-1993)
复制标题
DOI：
10.1099/0022-1317-80-7-1691
复制
发表时间：
1999-07-01
影响因子：
3.8
通讯作者：
Walimbe, AM
中科院分区：
文献类型：
--
作者：
Arankalle, VA;Paranjape, S;Walimbe, AM
代表 1976 年至 1991 年期间流行性和散发性戊型肝炎病例的 17 个印度戊型肝炎病毒 (HEV) 分离株在 RNA 聚合酶 (RNAP) 区域进行了测序。还在开放阅读框 1 的非结构高变区对五个分离株进行了测序。仅对原型分离株对开放阅读框 2 和 3 进行了测序。在比较保守 RNAP 区域的所有可用序列的基础上，HEV 分离株被分为三种基因型，彼此差异 > 15%。基因型 I 包括非洲和亚洲分离株，而基因型 II 和 III 分别由墨西哥和美国分离株代表。基因型I进一步分为四个亚基因型。大多数印度分离株 (15/20) 以及缅甸和尼泊尔分离株属于基因型 IA，基因型 IB 包括来自中国、巴基斯坦和前苏联的 HEV 分离株以及 2/20 印度分离株，代表了迄今为止最古老的 (1976) HEV 测序。基因型 IC 包括两个非洲分离株，而 3/20 印度分离株形成基因型 ID。 HEV 基因组其他区域的核苷酸序列分析也将分离株置于相同的基因型中。两个印度城市经历了第二次 HEV 流行，间隔时间为：8 年和 10 年，发现的亚基因型发生了变化；从 IB (Ahm-76) 到 IA (Ahm-84) 以及从 IA (Kol-81) 到 ID (Kol-91)。然而，没有发现基因型发生重大变化。总体而言，HEV 基因型似乎在地理上是隔离的。
Seventeen Indian hepatitis E virus (HEV) isolates, representing epidemic and sporadic hepatitis E cases during 1976-1991, were sequenced in the RNA polymerase (RNAP) region. Five isolates were also sequenced in the non-structural hypervariable region of open reading frame 1. Open reading frames 2 and 3 were sequenced only for the prototype isolate. On the basis of the comparison of all the available sequences of the conserved RNAP region, the HEV isolates were divided into three genotypes, differing from each other by > 15%. Genotype I included African and Asian isolates, whereas II and III were represented by Mexican and US isolates, respectively. Genotype I was further divided into four sub-genotypes. The majority of the Indian isolates (15/20), along with the Burmese and Nepali isolates, belonged to genotype IA, Genotype IB included HEV isolates from China, Pakistan and the former USSR and 2/20 Indian isolates, which represented the oldest (1976) HEV sequenced so far. Genotype IC included both the African isolates, whereas 3/20 Indian isolates formed genotype ID. Nucleotide sequence analysis of other regions of the HEV genome also placed isolates in the same genotypes. Both the Indian cities experiencing second HEV epidemics, after intervals of: 8 and 10 years, showed shifts in the sub-genotypes found; from IB (Ahm-76) to IA (Ahm-84) and from IA (Kol-81) to ID (Kol-91). However, no major shift in the genotypes was noted. Overall, HEV genotypes appear to be segregated geographically.