Effects of therapeutic hypothermia on the gut microbiota and metabolome of infants suffering hypoxic-ischemic encephalopathy at birth.
Effects of therapeutic hypothermia on the gut microbiota and metabolome of infants suffering hypoxic-ischemic encephalopathy at birth.
复制标题
低温治疗对出生时患有缺氧缺血性脑病的婴儿肠道微生物群和代谢组的影响。
DOI：
10.1016/j.biocel.2017.08.017
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
C. Ryan
中科院分区：
文献类型：
--
作者：
C. Watkins;C. Watkins;K. Murphy;S. Yen;I. Carafa;I. Carafa;Eugene M. Dempsey;C. O’Shea;E. A. Vercoe;R. Ross;R. Ross;C. Stanton;C. Stanton;C. Ryan
围产期新生儿缺氧缺血性脑病（HIE）可导致以后生活中显著的神经功能缺陷。全身冷却（TBC）是一种用于治疗HIE的神经保护策略，已被证明可以减少癫痫发作并改善治疗婴儿的神经发育结果。然而，HIE/TBC对发育中的肠道微生物群组成和随后的代谢谱的影响知之甚少。招募10例HIE足月婴儿，在33.5°C下接受TBC治疗72 h。对照组由9名健康的足月婴儿组成。两组均于2岁时收集粪便样本，保存于- 20℃。采用16S rRNA扩增子Illumina测序确定肠道微生物群组成，1H NMR分析确定粪便水的代谢谱。与未冷却的健康对照组相比，HIE/TBC婴儿的肠道微生物群组成中拟杆菌的比例显着降低。α多样性测量结果显示，HIE/TBC婴儿组的微生物丰富度多样性显著低于对照组（Shannon指数，<0.05）。两组的肠道菌群组成和代谢谱存在很大的个体间差异。在MetaboAnalyst 3.0上对化合物进行初步主坐标分析和层次聚类分析表明，这两个婴儿群体的代谢谱没有明显的分离。这些结果表明，与健康婴儿相比，HIE/TBC婴儿在2岁时肠道微生物发育没有显著影响。据我们所知，这是首次报道出生时患有HIE/TBC的婴儿肠道微生物群组成和代谢谱的研究。
Neonatal hypoxic ischemic encephalopathy (HIE) in the perinatal period can lead to significant neurological deficits in later life. Total body cooling (TBC) is a neuroprotective strategy used in the treatment of HIE and has been shown to reduce seizures and improve neurodevelopmental outcomes in treated infants. Little is known, however, about the effects of HIE/TBC on the developing gut microbiota composition and subsequent metabolic profile. Ten term infants with HIE who received TBC at 33.5 °C for 72 h were recruited. A control group consisted of nine healthy full term infants. Faecal samples were collected from both groups at 2 years of age and stored at −20 °C. 16S rRNA amplicon Illumina sequencing was carried out to determine gut microbiota composition and 1H NMR analysis was performed to determine the metabolic profile of faecal water. The gut microbiota composition of the HIE/TBC infants were found to have significantly lower proportions of Bacteroides compared to the non-cooled healthy control group. Alpha diversity measures detected significantly lower diversity in microbial richness in the HIE/TBC infant group compared to the control infants (Shannon index, <0.05). High inter-individual variation was found in gut microbiota composition and metabolic profile of both groups. Initial principal coordinate analysis and hierarchal clustering of compounds on MetaboAnalyst 3.0 indicated no clear separation in the metabolic profile of these two infant groups. These results suggest that there is no significant impact on the gut microbial development of HIE/TBC infants compared to healthy infants at 2 years of life. To our knowledge this is the first study to report the gut microbiota composition and metabolic profile of infants who have experienced HIE/TBC at birth.
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
6.1
作者：
Relman DA
通讯作者：
Relman DA
出生窒息会改变足月新生儿的肠道蠕动。
影响因子：
8
作者：
Berseth,CL;McCoy,HH
通讯作者：
McCoy,HH