Electrostatic adsorption of ferritin, proteins and nanoparticle conjugate onto the surface of polyelectrolyte multilayers
Electrostatic adsorption of ferritin, proteins and nanoparticle conjugate onto the surface of polyelectrolyte multilayers
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DOI：
10.1039/b807178k
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发表时间：
2008-08
影响因子：
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通讯作者：
K. Uto;Kazuya Yamamoto;N. Kishimoto;M. Muraoka;T. Aoyagi;I. Yamashita
中科院分区：
文献类型：
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作者：
K. Uto;Kazuya Yamamoto;N. Kishimoto;M. Muraoka;T. Aoyagi;I. Yamashita
本研究利用QCM、扫描电子显微镜和原子力显微镜对铁蛋白和载脂蛋白在前驱体薄膜表面的静电吸附进行了定量和动力学分析。通过交替吸附聚二烯丙基二甲基氯化铵(PDDA)和聚4-苯磺酸钠(PSS)，在合适浓度的氯化钠存在下，在QCM衬底上制备了前驱体薄膜。通过QCM的频移观察了它们的形成过程。此外，我们还通过QCM观察到(Apo)铁蛋白通过静电相互作用在最外层表面的吸附。(Apo)铁蛋白的吸附行为揭示了(Apo)铁蛋白的特殊吸附行为，即(Apo)铁蛋白只识别前驱体膜的表面，依赖于其表面电荷才能吸附到它上面。我们还研究了(Apo)铁蛋白溶液浓度、pH和洗涤/干燥工艺对其吸附行为的影响。本论文对蛋白质超分子的固定化研究将为构建更复杂的功能无机纳米结构提供一种方法。
In this study, we quantitatively and kinetically analyzed the electrostatic adsorption of ferritin and apoferritin onto the surface of precursor films by using QCM, SEM and AFM. The precursor films were successively prepared by the alternate adsorption of poly(diallyldimethylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) in the presence of suitable NaCl concentrations onto the QCM substrate. We observed their formation processes by frequency shifts of QCM. Furthermore, we also observed the adsorption of (apo)ferritin by an electrostatic interaction onto the outermost layer surface by QCM. The adsorption behavior of (apo)ferritin revealed the specific adsorption that (apo)ferritin only recognizes the surface of a precursor film depending on its surface charges in order to adsorb onto it. We also studied the effects of (apo)ferritin solution concentration, pH and washing/drying process on their adsorption behavior. This study on the immobilization of protein supramolecules will provide a method to build more complex functional inorganic nanostructures.