Effect of Salinity on DMSP Production in Gambierdiscus belizeanus (Dinophyceae)
Effect of Salinity on DMSP Production in Gambierdiscus belizeanus (Dinophyceae)
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DOI：
10.1111/jpy.12923
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发表时间：
2019-10-16
影响因子：
2.9
通讯作者：
Kiene, Ronald P.
中科院分区：
文献类型：
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作者：
Gwinn, Jessica K.;Robertson, Alison;Kiene, Ronald P.
二甲基磺基丙酸盐（DMSP）由许多海洋浮游植物产生，据报道具有多种有益功能，包括渗透调节。甲藻被认为是DMSP的主要生产者;然而，在这一群体中的积累情况差异很大。我们探讨了低盐度之间的生态相关的盐度（36和31）对DMSP积累，叶绿素a，细胞生长和细胞体积，超过12天的影响，在Gambierdiscus belizeanus。结果表明，G. belizeanus保持细胞内DMSP含量为16.3 pmol细胞（-1）和浓度为139 mM的两种盐度。虽然该细胞内浓度接近其它甲藻的中值，但G. belizeanus是迄今为止报告的所有甲藻中最高的，主要是因为它的体积大。DMSP水平没有显着影响盐度处理，但随着时间的推移，在实验过程中发生变化。盐度对DMSP：Chl a的比值有显著影响，表明盐度对G. belizeanus诱导的生理反应以外的DMSP的调整。一项对多种冈比亚盘属物种和品系中DMSP含量的调查显示，DMSP浓度相对较高（1.0-16.4 pmol细胞（-1）），属内和种内变异也较高。我们的结论是，虽然DMSP可能不参与该物种的长期（3-12天）的调节，G。belizeanus和其他Gambierdiscus物种可能是热带底栖微藻群落DMSP生产的重要贡献者，因为它们的体积大，细胞含量高。
Dimethylsulfoniopropionate (DMSP) is produced by many species of marine phytoplankton and has been reported to provide a variety of beneficial functions including osmoregulation. Dinoflagellates are recognized as major DMSP producers; however, accumulation has been shown to be highly variable in this group. We explored the effect of hyposaline transfer in Gambierdiscus belizeanus between ecologically relevant salinities (36 and 31) on DMSP accumulation, Chl a, cell growth, and cell volume, over 12 d. Our results showed that G. belizeanus maintained an intracellular DMSP content of 16.3 pmol cell(-1) and concentration of 139 mM in both salinities. Although this intracellular concentration was near the median reported for other dinoflagellates, the cellular content achieved by G. belizeanus was the highest reported of any dinoflagellate thus far, owing mainly to its large size. DMSP levels were not significantly affected by salinity treatment but did change over time during the experiment. Salinity, however, did have a significant effect on the ratio of DMSP:Chl a, suggesting that salinity transfer of G. belizeanus induced a physiological response other than DMSP adjustment. A survey of DMSP content in a variety of Gambierdiscus species and strains revealed relatively high DMSP concentrations (1.0-16.4 pmol cell(-1)) as well as high intrageneric and intraspecific variation. We conclude that, although DMSP may not be involved in long-term (3-12 d) osmoregulation in this species, G. belizeanus and other Gambierdiscus species may be important contributors to DMSP production in tropical benthic microalgal communities due to their large size and high cellular content.