Growth factor-defined culture medium for human mesenchymal stem cells
Growth factor-defined culture medium for human mesenchymal stem cells
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DOI：
10.1387/ijdb.103232sm
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发表时间：
2011-01-01
影响因子：
0.7
通讯作者：
Furue, Miho K.
中科院分区：
文献类型：
--
作者：
Mimura, Sumiyo;Kimura, Naohiro;Furue, Miho K.
人骨髓间充质干细胞（hMSCs）是治疗性干细胞的潜在细胞来源，因为它们具有增殖和分化成广泛的间充质细胞类型的能力，如成骨细胞、成软骨细胞和脂肪细胞。hMSCs已在临床上用于治疗移植物抗宿主病、成骨不全或牙槽裂患者，表明hMSCs移植作为基于干细胞的治疗相对安全。然而，用于hMSC的常规培养基含有胎牛血清（FBS）。在本研究中，我们开发了一种生长因子定义的，无血清的培养hMSCs的培养基。在这些条件下，TGF-β 1促进hMSCs的增殖。扩增的hMSC群体表达人多能性标志物SSEA-3、SSEA-4、NANOG、OCT 3/4和SOX 2。此外，在群体中检测到SSEA-3和间充质细胞标志物CD 105的双阳性细胞。证实了分化为成骨细胞和脂肪细胞的潜力。这项工作提供了一个有用的工具，以了解在文化中的hMSCs的基本生物学特性。
Human bone marrow-derived mesenchymal stem cells (hMSCs) are potential cellular sources of therapeutic stem cells as they have the ability to proliferate and differentiate into a wide array of mesenchymal cell types such as osteoblasts, chondroblasts and adipocytes. hMSCs have been used clinically to treat patients with graft vs. host disease, osteogenesis imperfect, or alveolar cleft, suggesting that transplantation of hMSCs is comparatively safe as a stem cell-based therapy. However, conventional culture medium for hMSCs contains fetal bovine serum (FBS). In the present study, we developed a growth factor-defined, serum-free medium for culturing hMSCs. Under these conditions, TGF-beta 1 promoted proliferation of hMSCs. The expanded hMSC population expressed the human pluripotency markers SSEA-3, -4, NANOG, OCT3/4 and SOX2. Furthermore, double positive cells for SSEA-3 and a mesenchymal cell marker, CD105, were detected in the population. The potential to differentiate into osteoblasts and adipocytes was confirmed. This work provides a useful tool to understand the basic biological properties of hMSCs in culture.