Isolation and characterization of the bacteriophage WO from Wolbachia, an arthropod endosymbiont
Isolation and characterization of the bacteriophage WO from Wolbachia, an arthropod endosymbiont
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DOI：
10.1016/j.bbrc.2004.03.164
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发表时间：
2004-05-14
影响因子：
3.1
通讯作者：
Sasaki, T
中科院分区：
文献类型：
--
作者：
Fujii, Y;Kubo, T;Sasaki, T
沃尔巴克氏体是一组专性共生细菌，在许多昆虫和其他节肢动物中发现。沃尔巴克氏体的存在改变了宿主的繁殖，但其机制尚不清楚。Wolbachia的分子生物学研究已经明显延迟，原因之一是缺乏这种细菌的转化技术。本研究首次从沃尔巴克氏体中分离到噬菌体颗粒。纯化的噬菌体具有直径约40 nm的等距头部，并含有约20 kbp的线性双链DNA。部分序列信息（共20，484bp）显示有24个开放阅读框，包括结构基因模块、复制基因和溶原性转化基因。这种噬菌体是唯一已知的可能用于转化沃尔巴克氏体的移动的遗传元件。(C)2004年爱思唯尔公司All rights reserved.
Wolbachia is a group of obligate symbiotic bacteria found in many insects and other arthropods. The presence of Wolbachia alters reproduction in the host, but the mechanisms are unknown. Molecular biological studies of Wolbachia have delayed significantly, and one of the reasons is the lack of transformation techniques of this bacterium. In the present study, bacteriophage particles were isolated from Wolbachia for the first time. The purified phage had an isometric head that was approximately 40 nm, in diameter and contained linear double-stranded DNA of approximately 20kbp. Partial sequence information (total of 20,484bp) revealed that there were 24 open reading frames including a structural gene module, and genes for replication and lysogenic conversion. This bacteriophage is the only known mobile genetic element potentially used for transformation of Wolbachia. (C) 2004 Elsevier Inc. All rights reserved.