CDNA cloning and expression of bacteria-induced Hdd11 gene from eri-silkworm, Samia cynthia ricini
CDNA cloning and expression of bacteria-induced Hdd11 gene from eri-silkworm, Samia cynthia ricini
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DOI：
10.1016/j.cca.2003.10.007
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发表时间：
2003-12-01
影响因子：
3.9
通讯作者：
Morishima, I
中科院分区：
文献类型：
--
作者：
Bao, YY;Mega, K;Morishima, I
利用抑制性消减杂交技术从蓖麻蚕幼虫脂肪体中克隆了一个编码细菌诱导蛋白Hdd11的cDNA克隆。该cDNA编码一个167个氨基酸残基的开放阅读框架，其中包含一个18个氨基酸残基的预测信号肽。推导的氨基酸序列与美国白蛾和烟草天蛾Hdd11蛋白的同源性分别为54%和55%。北方印迹杂交检测时，该基因的表达是不可检测的幼稚幼虫，但强烈诱导脂肪体注射细菌细胞或肽聚糖到幼虫。脂肪体中的mRNA表达早在注射后3 h就被检测到，在12 h达到峰值水平，并以显著水平持续另外60 h。在中肠、血细胞和马氏管中检测到非常低水平的转录本。(C)2003年爱思唯尔公司All rights reserved.
A cDNA clone encoding Hdd11 protein, a bacteria-induced protein of unknown function, was isolated from fat body of immunized Samia cynthia ricini larvae based on suppression subtractive hybridization. The cDNA encodes a 167 amino acid residue open reading frame with an 18 residue predicted signal peptide. The deduced amino acid sequence showed 54% and 55% identity with Hdd11 proteins from Hyphantria cunea and Manduca sexta, respectively. Expression of the gene was undetectable in naive larvae when measured by Northern blot hybridization, but strongly induced in fat body after injecting bacterial cells or peptidoglycan into the larvae. The mRNA expression in the fat body was detected as early as 3 h post injection, reached peak levels at 12 h and continued for further 60 h at significant levels. The transcript was detected at very low levels in midgut, hemocytes and malpighian tubules. (C) 2003 Elsevier Inc. All rights reserved.