ANP and BNP Responses to Dehydration in the One-Humped Camel and Effects of Blocking the Renin-Angiotensin System
ANP and BNP Responses to Dehydration in the One-Humped Camel and Effects of Blocking the Renin-Angiotensin System
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DOI：
10.1371/journal.pone.0057806
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发表时间：
2013-03-13
期刊：
影响因子：
3.7
通讯作者：
Kazzam, Elsadig
中科院分区：
文献类型：
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作者：
Adem, Abdu;Al Haj, Mahmoud;Kazzam, Elsadig
本研究的目的是调查和比较心钠素（ANP）和B型利钠肽（BNP）在循环中的反应，水合，脱水和脱水氯沙坦治疗的骆驼;并记录在骆驼心脏中的心脏存储形式的B型利钠肽。在研究中使用了18只雄性骆驼：对照或水合骆驼（n = 6）、脱水骆驼（n = 6）和脱水氯沙坦治疗骆驼（n = 6），所述脱水骆驼脱水并以5 mg/kg体重的剂量静脉内接受血管紧张素II（Ang II）AT-1受体阻断剂氯沙坦20天。对照组动物自由采食饲料和水，而脱水和脱水氯沙坦治疗组动物自由采食饲料，但不饮水，持续20天。与时间匹配的对照组相比，脱水骆驼的血浆ANP和BNP水平显着下降。氯沙坦治疗的骆驼在20天的脱水过程中也表现出ANP和BNP水平的显著下降，但这些变化与单独脱水时观察到的变化没有差异。体积排阻高效液相色谱分析表明，脑钠肽前体是该肽的贮存形式。我们的结论是：第一，在骆驼脱水诱导大力递减循环水平的ANP和BNP;第二，阻断肾素-血管紧张素系统有很少或没有调节作用的ANP和BNP响应脱水;第三，前B型利钠肽是这种激素的存储形式在心脏的单峰骆驼。
The objectives of this study were to investigate and compare the responses of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) in the circulation of hydrated, dehydrated, and dehydrated losartan - treated camels; and to document the cardiac storage form of B-type natriuretic peptide in the camel heart. Eighteen male camels were used in the study: control or hydrated camels (n = 6), dehydrated camels (n = 6) and dehydrated losartan-treated camels (n = 6) which were dehydrated and received the angiotensin II (Ang II) AT-1 receptor blocker, losartan, at a dose of 5 mg/kg body weight intravenously for 20 days. Control animals were supplied with feed and water ad-libitum while both dehydrated and dehydrated-losartan treated groups were supplied with feed ad-libitum but no water for 20 days. Compared with time-matched controls, dehydrated camels exhibited a significant decrease in plasma levels of both ANP and BNP. Losartan-treated camels also exhibited a significant decline in ANP and BNP levels across 20 days of dehydration but the changes were not different from those seen with dehydration alone. Size exclusion high performance liquid chromatography of extracts of camel heart indicated that proB-type natriuretic peptide is the storage form of the peptide. We conclude first, that dehydration in the camel induces vigorous decrements in circulating levels of ANP and BNP; second, blockade of the renin-angiotensin system has little or no modulatory effect on the ANP and BNP responses to dehydration; third, proB-type natriuretic peptide is the storage form of this hormone in the heart of the one-humped camel.