Targeted, multimodality PET-CT and optical imaging platform for visualizing biological function.
Targeted, multimodality PET-CT and optical imaging platform for visualizing biological function.
复制标题
用于可视化生物功能的靶向多模态 PET-CT 和光学成像平台。
DOI：
10.1053/j.gastro.2010.09.030
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发表时间：
2010
期刊：
影响因子：
29.4
通讯作者：
Wang,ThomasD
中科院分区：
文献类型：
--
作者：
Wang,ThomasD
In this paper, Nahrendorf et al. demonstrate a multi-modality imaging platform that combines the use of nuclear (positron emission tomography, PET), anatomic (computed tomography, CT), and optical (fluorescence-mediated tomography, FMT). The performance of this integrated imaging system was demonstrated with detection and localization of implanted colon cancer lesions in a mouse model. This study was performed using an imaging probe based on a dextran-coated (cross-linked iron oxide, CLIO) nanoparticle that accumulates preferentially in macrophages. These nanoparticles are dual-labeled with either fluorine-18 (18 F) or copper-64 (64 Cu), short half-life positron emitting radioisotopes, and with a far red fluorescent dye (VT680). First, linearity and the minimum detection thresholds of PET (Siemens Inveon) and optical (VisEn Medical FMT-2500) imaging over a range of concentrations for the imaging agents, 18 F-CLIO-VT680 and 64 Cu-CLIO-VT680, were evaluated using a phantom that consisted of serial dilutions of the probes in agar. Image z-stacks were collected and volume rendered to produce 3-dimensional images, and excellent correlation (r 2> 0.99) between the PET and optical signals was found. The PET and optical images were then converted into a standard (DICOM) format, and 3-dimensional surface rendered reconstructions of the PET and optical images were co-registered using imaging landmarks and fiducial markers. Congruence between these images in the xy-plane was demonstrated and quantified with a cross-correlation function (CCF) based on Pearson's coefficient. A maximum correlation coefficient of the 2-dimensional signal of 0.85 was found.
