Light-Dark Modulates Root Hydrotropism Associated with Gravitropism by Involving Amyloplast Response in Arabidopsis
Light-Dark Modulates Root Hydrotropism Associated with Gravitropism by Involving Amyloplast Response in Arabidopsis
复制标题
光暗通过涉及拟南芥中的淀粉体反应来调节与向地性相关的根向水性
DOI：
10.1016/j.celrep.2020.108198
复制
发表时间：
2020-09-29
期刊：
影响因子：
8.8
通讯作者：
Xu, Weifeng
中科院分区：
文献类型：
--
作者：
Li, Ying;Yuan, Wei;Xu, Weifeng
淀粉质体在亲水性和向重力性之间的相互作用已经被描述过了。然而，明暗对这两种取向之间相互作用的影响尚不清楚。在这里，通过开发一种方法，使其能够比传统的实验室条件更接近地模拟自然条件，我们表明，与完全暴露在光下的幼苗相比，在其芽被照亮但其根在黑暗中生长的野生型拟南芥幼苗中，亲水性更强。由于根尖淀粉体含量的减少和淀粉生物合成关键基因PGM1基因表达的降低，根系向重力性显著降低，这可能是黑暗条件下增强根系向水性的原因之一。此外，与野生型相比，淀粉缺乏突变体PGM1-1表现出更强的亲水性。我们的结果表明，在光-暗调制下，拟南芥的淀粉体反应和淀粉减少，其次是向重力性降低和向水性增强。
The role of amyloplasts in the interactions between hydrotropism and gravitropism has been previously described. However, the effect of light-dark on the interactions between the two tropisms remains unclear. Here, by developing a method that makes it possible to mimic natural conditions more closely than the conventional lab conditions, we show that hydrotropism is higher in wild-type Arabidopsis seedlings whose shoots are illuminated but whose roots are grown in the dark compared with seedlings that are fully exposed to light. Root gravitropism is substantially decreased because of the reduction of amyloplast content in the root tip with decreased gene expression in PGM1 (a key starch biosynthesis gene), which may contribute to enhanced root hydrotropism under darkness. Furthermore, the starch-deficient mutant pgm1-1 exhibits greater hydrotropism compared with wild-type. Our results suggest that amyloplast response and starch reduction occur under light-dark modulation, followed by decreased gravitropism and enhanced hydrotropism in Arabidopsis root.