MUCOUS SECRETION IN RAT COLONIC MUCOSA DURING CARCINOGENESIS INDUCED BY DIMETHYLHYDRAZINE - MORPHOLOGICAL AND HISTOCHEMICAL STUDY
MUCOUS SECRETION IN RAT COLONIC MUCOSA DURING CARCINOGENESIS INDUCED BY DIMETHYLHYDRAZINE - MORPHOLOGICAL AND HISTOCHEMICAL STUDY
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DOI：
10.1038/bjc.1975.134
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发表时间：
1975-01-01
影响因子：
8.8
通讯作者：
FILIPE, MI
中科院分区：
文献类型：
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作者：
FILIPE, MI
我们以前的研究，在大肠癌切除标本中，已经显示了在表面上”正常”的粘膜区域中粘液分泌的异常模式，其中杯状细胞主要产生唾液酸粘蛋白，而真正的正常结肠粘膜中以硫粘蛋白为主。本文用1，2-二甲基肼-2HCl（DMH）诱发大鼠大肠癌。我们试图研究癌变过程中结肠上皮发生的组织学和分泌异常，并将这些变化与上述人类变化相关联。在DMH处理的大鼠的结肠粘膜中观察到的显微镜和组织学病变证实了其他作者的发现，并且类似于人类结肠直肠癌。检测到的最早变化是小的增生灶，从第6周开始伴有几个异型增生灶。第15周出现原位癌，第19周开始出现浸润癌。在大多数显示轻度至中度异型增生的区域中观察到粘液分泌类型的变化（唾液粘蛋白占优势），而周围正常上皮产生硫酸化物质。粘膜耗竭是一个共同的特点，在严重的异型增生和癌的领域。这些发现与在人结肠粘膜癌中观察到的粘蛋白组成的相似变化相关，进一步支持了我们先前的假设，即以唾液酸粘蛋白增加为特征的粘蛋白变化可能反映了早期恶性转化。如果这一假设被证明是正确的，使用一种简单的方法来鉴定大肠活检中的粘蛋白将对发现早期恶性肿瘤有很大的帮助。
Our previous studies, in specimens of large intestine resected for carcinoma, have shown abnormal patterns of mucous secretion in areas of apparently" normal" mucosa, where goblet cells produce mainly sialomucins as compared with the true normal colonic mucosa in which sulphomucins predominate. In the present work, large bowel cancer was induced in rats by the administration of 1, 2-dimethylhydrazine-2HCl (DMH). We attempted to study the sequential histological and secretory abnormalities which developed in the colonic epithelium during carcinogenesis, and to correlate these changes with those described above in the human. The microscopical and histological lesions observed in the colonic mucosa of DMH treated rats confirmed the findings of other authors and resembled the human colorectal cancer. The earliest changes detected were small foci of hyperplasia accompanied from the 6th week onwards by several foci of dysplasia. Carcinoma in situ appeared at the 15th week and finally invasive carcinoma developed from the 19th week onwards. Changes in the type of mucous secretion, with predominance of sialomucins, were observed in the majority of the areas showing mild to moderate dysplasia whilst the surrounding normal epithelium produced suphated material. Mucous depletion was a common feature in areas of severe dysplasia and carcinoma. These findings correlated well with the similar variations in the mucin composition observed in human colonic mucosa in carcinoma and further supported our previous hypothesis that mucin changes characterized by an increase in sialomucins might reflect early malignant transformation. If this hypothesis proved to be correct, the use of a simple method for the identification of mucins in large bowel biopsies would be of great help in detecting early malignancy.