Dopamine-functionalized cyclodextrins: modification of reduced graphene oxide based electrodes and sensing of folic acid in human serum
Dopamine-functionalized cyclodextrins: modification of reduced graphene oxide based electrodes and sensing of folic acid in human serum
复制标题
DOI：
10.1007/s00216-019-01892-1
复制
发表时间：
2019-08-01
影响因子：
4.3
通讯作者：
Szunerits, Sabine
中科院分区：
文献类型：
--
作者：
Chekin, Fereshteh;Mishyn, Vladyslav;Szunerits, Sabine
任何传感器制造中的一个强制性步骤是在传感器表面引入分析物特定的识别元件。在这项研究中，证明了将β-环糊精修饰的多巴胺固定在还原的氧化石墨烯电化学传感器上用于叶酸的灵敏和选择性传感的可能性。该传感器对叶酸的氧化表现出良好的电催化活性。由于表面限制的β-环糊精对叶酸具有很强的亲和力，加上良好的电子转移特性，使得该传感器对叶酸的检测下限为1 nM，线性响应高达10微米。对健康人和叶酸缺乏症患者的血清样本的测试验证了传感器的能力。
A mandatory step in any sensor fabrication is the introduction of analyte-specific recognition elements to the transducer surface. In this study, the possibility to anchor beta-cyclodextrin-modified dopamine to a reduced graphene oxide based electrochemical transducer for the sensitive and selective sensing of folic acid is demonstrated. The sensor displays good electrocatalytic activity toward the oxidation of folic acid. The strong affinity of the surface-confined beta-cyclodextrin for folic acid, together with favorable electron transfer characteristics, resulted in a sensor with a detection limit of 1 nM for folic acid and a linear response up to 10 mu M. Testing of the sensor on serum samples from healthy individuals and patients diagnosed with folic acid deficiency validated the sensing capability.