The age of enlightenment: evolving opportunities in brain research through optical manipulation of neuronal activity.
The age of enlightenment: evolving opportunities in brain research through optical manipulation of neuronal activity.
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DOI：
10.3389/fnsys.2011.00095
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发表时间：
2011
影响因子：
3
通讯作者：
Heck DH
中科院分区：
文献类型：
--
作者：
Jerome J;Heck DH
神经元活动的光学操纵已经迅速发展成为在一系列尺度上研究与神经元连接相关的机制的最强大和最广泛使用的方法。由于早期使用单位点解锁来映射网络连接，光调制技术的快速技术发展增加了重要的新选项，例如快速扫描光刺激、光刺激的大规模并行控制、全息解锁和双光子刺激技术。光遗传学令人兴奋的新发展通过提供细胞类型特异性和体内可用性，提供对行为神经基质的光学访问，补充了神经递质释放技术。在这里，我们回顾了神经元活动的光学操纵方法的快速发展，强调了至关重要的最新进展。
Optical manipulation of neuronal activity has rapidly developed into the most powerful and widely used approach to study mechanisms related to neuronal connectivity over a range of scales. Since the early use of single site uncaging to map network connectivity, rapid technological development of light modulation techniques has added important new options, such as fast scanning photostimulation, massively parallel control of light stimuli, holographic uncaging, and two-photon stimulation techniques. Exciting new developments in optogenetics complement neurotransmitter uncaging techniques by providing cell-type specificity and in vivo usability, providing optical access to the neural substrates of behavior. Here we review the rapid evolution of methods for the optical manipulation of neuronal activity, emphasizing crucial recent developments.