Successful stabilization of graphene oxide in electrolyte solutions: enhancement of biofunctionalization and cellular uptake.
Successful stabilization of graphene oxide in electrolyte solutions: enhancement of biofunctionalization and cellular uptake.
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DOI：
10.1021/nn202355p
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发表时间：
2012-01-24
期刊：
影响因子：
17.1
通讯作者：
Nguyen, SonBinh T.
中科院分区：
文献类型：
--
作者：
Hong, Bong Jin;Compton, Owen C.;An, Zhi;Eryazici, Ibrahim;Nguyen, SonBinh T.
关键词：
在电解质存在的情况下，氧化石墨烯的水分散体本身是不稳定的，电解质会屏蔽这些纳米片上的静电表面电荷并导致不可逆的聚集。已经开发了两种互补的策略，即利用静电稳定或空间位阻稳定，来提高氧化石墨烯在电解质溶液中的稳定性，使其能够在细胞培养基和血清中保持分散。静电稳定方法需要将氧化石墨烯进一步氧化至低C/O比（约1.03），并提高这些纳米片的离子耐受性。空间位阻稳定技术采用一种两亲性嵌段共聚物作为非共价结合的表面活性剂，以最小化由聚集引起的纳米片 - 纳米片相互作用。这两种策略都可以在生物介质中稳定大尺寸（>300 nm）的氧化石墨烯纳米片，与未处理的纳米片相比，链霉亲和素的生物共轭效率提高了>250%。值得注意的是，这两种策略都能使稳定的纳米片容易被细胞摄取，这表明它们作为潜在的药物递送载体具有优异的性能。
Aqueous dispersions of graphene oxide are inherently unstable in the presence of electrolytes, which screen the electrostatic surface charge on these nanosheets and induce irreversible aggregation. Two complementary strategies, utilizing either electrostatic or steric stabilization, have been developed to enhance the stability of graphene oxide in electrolyte solutions, allowing it to stay dispersed in cell culture media and serum. The electrostatic stabilization approach entails further oxidation of graphene oxide to low C/O ratio (~1.03) and increases ionic tolerance of these nanosheets. The steric stabilization technique employs an amphiphilic block copolymer that serves as a non-covalently bound surfactant to minimize the aggregate-induced nanosheets-nanosheet interactions. Both strategies can stabilize graphene oxide nanosheets with large dimensions (>300 nm) in biological media, allowing for an enhancement of >250% in the bioconjugation efficiency of streptavidin in comparison to untreated nanosheets. Notably, both strategies allow the stabilized nanosheets to be readily uptake by cells, demonstrating their excellent performance as potential drug delivery vehicles.
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