Characterization of a receptor-like protein for 1,25-dihydroxyvitamin D3 in rat skin.
Characterization of a receptor-like protein for 1,25-dihydroxyvitamin D3 in rat skin.
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大鼠皮肤中 1,25-二羟基维生素 D3 受体样蛋白的表征。
DOI：
10.1073/pnas.77.10.5822
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发表时间：
1980
影响因子：
11.1
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Simpson,RU;DeLuca,HF
离体大鼠表皮具有1，25-二羟维生素D3的胞质3.5 S受体样蛋白。这种3.5S结合剂对1，25-二羟基维生素D3具有高亲和力（Kd = 1.4 × 10 - 10 M），并且以低浓度（每mg胞质溶胶蛋白31 fmol结合位点）存在。受体结合的类似物竞争揭示了以下效力顺序：1，25-二羟基维生素D3 > 25-羟基维生素D3 > 1 α-羟基维生素D3 > 24（R），25-二羟基维生素D3 >维生素D3。该受体的分子量为60，000，对DNA-纤维素具有亲和力，在低钾浓度下聚集。1，25-二羟维生素D3结合剂通过硫酸氢钠（10 mM）稳定。发现添加苯甲基磺酰氟（3 mM）产生更可再现的受体制剂。1，25-二羟维生素D3结合大分子的存在与骨和肠受体的性质相似，表明皮肤是这种激素的另一个靶器官。
Isolated rat epidermis possesses a cytosolic 3.5 S receptor-like protein for 1,25-dihydroxyvitamin D3. This 3.5S binder has a high affinity (Kd = 1.4 X 10(-10) M) for 1,25-dihydroxyvitamin D3 and is present in low concentrations (31 fmol of binding sites per mg of cytosol protein). Analog competition for receptor binding revealed the following potency order: 1,25-dihydroxyvitamin D3 > 25-hydroxyvitamin D3 > 1 alpha-hydroxyvitamin D3 > 24 (R),25-dihydroxyvitamin D3 > vitamin D3. The receptor has a molecular weight of 60,000, has affinity for DNA-cellulose, and aggregates in the presence of low potassium concentrations. The 1,25-dihydroxyvitamin D3 binder is stabilized by sodium molybdate (10 mM). Addition of phenylmethylsulfonyl fluoride (3 mM) was found to yield more reproducible receptor preparations. The presence of a 1,25-dihydroxyvitamin D3 binding macromolecule with properties similar to those of the bone and intestinal receptor suggests that skin is an additional target organ for this hormone.