Determination of the in vivo prebiotic potential of a maize-based whole grain breakfast cereal: a human feeding study
Determination of the in vivo prebiotic potential of a maize-based whole grain breakfast cereal: a human feeding study
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DOI：
10.1017/s0007114510002084
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发表时间：
2010-11-01
影响因子：
3.6
通讯作者：
Tuohy, Kieran M.
中科院分区：
文献类型：
--
作者：
Carvalho-Wells, Andrew L.;Helmolz, Kathrin;Tuohy, Kieran M.
流行病学研究表明，心血管疾病的风险和摄入全谷物（WG）丰富的食物之间呈反比关系。经常食用早餐谷物不仅可以增加饮食中的WG，还可以改善心血管健康。已经提出了各种机制，包括结肠微生物群的益生元调节。在本研究中，在一项双盲、安慰剂对照的人类喂养研究（n 32）中评估了玉米衍生WG谷物（WGM）的益生元活性。在21天的时间里，健康男性和女性，平均年龄32（SD 8）岁，BMI 23.3（SD 0.58）kg/m2，以交叉方式消耗48 g/d WG谷物（WGM）或48 g安慰剂谷物（非全谷物（NWG））。在研究期间第0、21、42、63和84天的5个时间点（代表基线、两次治疗后和两个洗脱期）采集粪便样本。使用对拟杆菌属特异性的16 S rRNA寡核苷酸探针进行荧光原位杂交，双歧杆菌属，溶组织梭菌/产气荚膜梭菌亚群、乳酸杆菌-肠球菌亚群和细菌总数。在食用WGM 21天后，与对照谷物相比，粪便双歧杆菌的平均组水平显著增加（P=0.001）。经过3周的洗脱期后，双歧杆菌水平恢复到干预前的水平。血清脂质、葡萄糖或粪便排出量指标未观察到统计学显着变化。总之，这种富含WG玉米的早餐谷物介导了肠道微生物群的生物合成调节，表明可能的益生元作用模式。
Epidemiological studies have shown an inverse relationship between risk of CVD and intake of whole grain (WG)-rich food. Regular consumption of breakfast cereals can provide not only an increase in dietary WG but also improvements to cardiovascular health. Various mechanisms have been proposed, including prebiotic modulation of the colonic microbiota. In the present study, the prebiotic activity of a maize-derived WG cereal (WGM) was evaluated in a double-blind, placebo-controlled human feeding study (n 32). For a period of 21d, healthy men and women, mean age 32 (SD 8) years and BMI 23.3 (SD 0.58) kg/m(2), consumed either 48 g/d WG cereal (WGM) or 48 g placebo cereal (non-whole grain (NWG)) in a crossover fashion. Faecal samples were collected at five points during the study on days 0, 21, 42, 63 and 84 (representing at baseline, after both treatments and both wash-out periods). Faecal bacteriology was assessed using fluorescence in situ hybridisation with 16S rRNA oligonucleotide probes specific for Bacteroides spp., Bifidobacterium spp., Clostridium histolyticum/perfringens subgroup, Lactobacillus-Enterococcus subgroup and total bacteria. After 21d consumption of WGM, mean group levels of faecal bifidobacteria increased significantly compared with the control cereal (P=0.001). After a 3-week wash-out period, bifidobacterial levels returned to pre-intervention levels. No statistically significant changes were observed in serum lipids, glucose or measures of faecal output. In conclusion, this WG maize-enriched breakfast cereal mediated a bifidogenic modulation of the gut microbiota, indicating a possible prebiotic mode of action.