Biomolecule immobilization techniques for bioactive paper fabrication
Biomolecule immobilization techniques for bioactive paper fabrication
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DOI：
10.1007/s00216-012-5821-1
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发表时间：
2012-04-01
影响因子：
4.3
通讯作者：
Hu, Yim Fun
中科院分区：
文献类型：
--
作者：
Kong, Fanzhi;Hu, Yim Fun
对纸基传感器或具有主动识别能力的分析功能的功能材料的研究正在迅速扩大，并且在生物传感器层面的生物活性纸的设计和制造上投入了大量的研究工作，以检测潜在的健康危害。制造生物活性纸的关键步骤是设计将抗体、酶、噬菌体、细胞、蛋白质、合成聚合物和 DNA 适体等生物分子固定在适当制备的纸膜上的实验和操作程序。固定化方法简单地分为物理吸收、生物活性墨水截留、生物亲和附着和共价化学键固定化。每种方法都有各自的固定特征。尽管每种生物分子-纸张组合在使用前都必须进行优化，但生物活性墨水包埋方法由于其普遍适用性和生物相容性而成为最常用的方法。目前，生物活性纸有四种常见应用：（1）用于样品调节的纸基生物测定或纸基分析装置； （二）包装、建筑行业的假冒伪劣行为； （3）食品和水质监测的病原菌检测； (4)使用抗菌纸灭活病原菌。本文回顾和比较了不同的生物分子固定技术并讨论了当前的趋势。还讨论了生物活性纸当前、新兴和未来的应用。 '
Research into paper-based sensors or functional materials that can perform analytical functions with active recognition capabilities is rapidly expanding, and significant research effort has been made into the design and fabrication of bioactive paper at the biosensor level to detect potential health hazards. A key step in the fabrication of bioactive paper is the design of the experimental and operational procedures for the immobilization of biomolecules such as antibodies, enzymes, phages, cells, proteins, synthetic polymers and DNA aptamers on a suitably prepared paper membrane. The immobilization methods are concisely categorized into physical absorption, bioactive ink entrapment, bioaffinity attachment and covalent chemical bonding immobilization. Each method has individual immobilization characteristics. Although every biomolecule-paper combination has to be optimized before use, the bioactive ink entrapment method is the most commonly used approach owing to its general applicability and biocompatibility. Currently, there are four common applications of bioactive paper: (1) paper-based bioassay or paper-based analytical devices for sample conditioning; (2) counterfeiting and countertempering in the packaging and construction industries; (3) pathogen detection for food and water quality monitoring; and (4) deactivation of pathogenic bacteria using antimicrobial paper. This article reviews and compares the different biomolecule immobilization techniques and discusses current trends. Current, emerging and future applications of bioactive paper are also discussed. '