Relationship between dynamic instability of individual microtubules and flux of subunits into and out of polymer
Relationship between dynamic instability of individual microtubules and flux of subunits into and out of polymer
复制标题
单个微管的动态不稳定性与进出聚合物的亚基通量之间的关系
DOI：
10.1002/cm.21557
复制
发表时间：
2019
期刊：
影响因子：
2.9
通讯作者：
Goodson, Holly V.
中科院分区：
文献类型：
--
作者：
Mauro, Ava J.;Jonasson, Erin M.;Goodson, Holly V.
动态聚合物如微管和肌动蛋白的行为经常在以下尺度中的一个或两个尺度上进行评估：（a）本体聚合物的净组装或拆卸，（B）单个细丝的生长和缩短。先前的工作已经推导出各种形式的方程来关联本体聚合物质量的变化速率（即，亚基流入和流出聚合物，通常缩写为“J”）到单个细丝行为。然而，这些版本的“Jequation”在用于量化单个细丝行为的变量方面有所不同，这对应于不同的实验方法。例如，J方程的某些变体使用动态不稳定性参数，通过长时间跟踪特定的单个细丝获得。该方程的另一种形式使用了许多个体在短时间内的测量结果。我们使用的推导和计算机模拟相结合，模仿实验，以（a）与各种形式的theJ方程彼此，（B）确定条件下，这些方程的形式是和不相等的，和（c）确定方面的测量，可以影响的准确性，每种形式的theJ方程。对J方程及其与实验可测量量的联系的进一步理解将有助于建立稳态聚合物行为的多尺度理解。
Behaviors of dynamic polymers such as microtubules and actin are frequently assessed at one or both of the following scales: (a) net assembly or disassembly of bulk polymer, (b) growth and shortening of individual filaments. Previous work has derived various forms of an equation to relate the rate of change in bulk polymer mass (i.e., flux of subunits into and out of polymer, often abbreviated as “J”) to individual filament behaviors. However, these versions of the “Jequation” differ in the variables used to quantify individual filament behavior, which correspond to different experimental approaches. For example, some variants of theJequation use dynamic instability parameters, obtained by following particular individual filaments for long periods of time. Another form of the equation uses measurements from many individuals followed over short time steps. We use a combination of derivations and computer simulations that mimic experiments to (a) relate the various forms of theJequation to each other, (b) determine conditions under which theseJequation forms are and are not equivalent, and (c) identify aspects of the measurements that can affect the accuracy of each form of theJequation. Improved understanding of theJequation and its connections to experimentally measurable quantities will contribute to efforts to build a multiscale understanding of steady‐state polymer behavior.