Identification of an urinary metabolite profile associated with osteoarthritis
Identification of an urinary metabolite profile associated with osteoarthritis
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DOI：
10.1016/j.joca.2005.04.005
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发表时间：
2005-09-01
影响因子：
7
通讯作者：
DeGroot, J
中科院分区：
文献类型：
--
作者：
Lamers, RJAN;van Nesselrooij, JHJ;DeGroot, J
目的：骨关节炎（Osteoarthritis，OA）是老年人最常见的疾病之一。主要特征是关节软骨的进行性破坏。我们缺乏定量和敏感的OA生物标志物来检测疾病早期关节的变化。在这项研究中，我们研究了是否可以发现尿代谢产物谱，可以作为诊断生物标志物的OA在人类。我们还比较了配置文件，我们以前在豚鼠自发CA model.Methods：尿液样本的92名参与者（47个非OA控制和45个人与放射照相CA的膝盖或臀部）被选中的约翰斯顿县骨关节炎项目（北卡罗来纳州，美国）。参与者的年龄从60岁到84岁不等。结果：OA患者尿液核磁共振谱与对照组相比存在差异（男性和女性均P < 0.001）。可以确定与OA密切相关的代谢产物谱。该特征在很大程度上类似于之前在患有CA的豚鼠中确定的特征（类似于人类特征的125个信号中的40个也存在于豚鼠特征中）。尿代谢产物谱与骨关节炎严重程度相关（R-2 = 0.82（男性）; R-2 = 0.93（女性））。结论：尿代谢产物谱可作为骨关节炎的一种新的生物标志物。(c)2005年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Osteoarthritis (OA) is one of the most common diseases among the elderly. The main characteristic is the progressive destruction of articular cartilage. We lack quantitative and sensitive biomarkers for OA to detect changes in the joints in an early stage of the disease. In this study, we investigated whether a urinary metabolite profile could be found that could serve as a diagnostic biomarker for OA in humans. We also compared the profile we obtained previously in the guinea pig spontaneous CA model.Methods: Urine samples of 92 participants (47 non-OA controls and 45 individuals with radiographic CA of the knees or hips) were selected from the Johnston County Osteoarthritis Project (North Carolina, USA). Participants ranged in age from 60 to 84 years. Samples were measured by H-1 nuclear magnetic resonance spectroscopy (NMR) with subsequent principal component discriminant analysis and partial least squares regression analysis.Results: Differences were observed between urine NMR spectra of OA cases and controls (P < 0.001 for both male and female subjects). A metabolite profile could be determined which was strongly associated with OA. This profile largely resembled the profile previously identified for guinea pigs with CA (similar to 40 out of the similar to 125 signals of the human profile were present in the guinea pig profile as well). A correlation was found between the metabolite profile and radiographic OA severity (R-2 = 0.82 (male); R-2 = 0.93 (female)).Conclusion: This study showed that a urine metabolite profile may serve as a novel discriminating biomarker of OA. (c) 2005 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.