Self-assembled nanomaterials based on aggregation-induced emission of AuNCs: Fluorescence and colorimetric dual-mode biosensing of organophosphorus pesticides
Self-assembled nanomaterials based on aggregation-induced emission of AuNCs: Fluorescence and colorimetric dual-mode biosensing of organophosphorus pesticides
复制标题
基于AuNCs聚集诱导发射的自组装纳米材料：有机磷农药的荧光和比色双模式生物传感
DOI：
10.1016/j.snb.2020.128481
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发表时间：
2020-10-15
影响因子：
8.4
通讯作者：
Liu, Yingju
中科院分区：
文献类型：
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作者：
Cai, Yue;Qiu, Ziyin;Liu, Yingju
有机磷农药在农业中应用广泛。然而，有机磷农药的残留和积累对食品安全和人体健康构成了威胁。因此，一种灵敏、准确的OPs检测方法仍有待探索。本研究利用静电吸附将弱荧光AuNCs固定在SiO2表面，激发其聚集诱导发射（AIE）特性，实现荧光信号的放大。在此基础上，构建了基于自组装MnO2-AuNCs-SiO2纳米复合材料的荧光比色传感器。抗坏血酸（AA）是l-抗坏血酸-2-磷酸钠（AAP）在碱性磷酸酶（ALP）存在下的产物，可以分解MnO2并“开启”荧光传感器。此外，由于OPs对ALP活性的抑制作用，建立了一种荧光-比色双模检测OPs的方法。荧光检测线性范围为1 ~ 50 mg/L，检出限为0.4 μ g/L；比色分析线性范围为0.1 ~ 50 mg/L，检出限为0.09 μ g/L。此外，还实现了新鲜蔬菜中OPs的原位成像，实现了试纸上OPs的视觉半定量检测。因此，荧光生物传感器在现场检测中具有潜在的应用价值。
Organophosphorus pesticides (OPs) are widely used in agriculture. However, the residue and accumulation of OPs is a threat to food safety and human health. Thus, a sensitive and accurate OPs detection method is still pursued. Here, we utilized electrostatic adsorption to immobilize weak fluorescent AuNCs on the SiO2 surface, exciting its aggregation-induced emission (AIE) properties and achieving the amplification of the fluorescent signal. Furthermore, a fluorescence-colorimetric sensor based on self-assembled MnO2-AuNCs-SiO2 nano composites was constructed. Ascorbic acid (AA), the product of sodium L-ascorbyl-2-phosphate (AAP) in the presence of alkaline phosphatase (ALP), can decompose MnO2 and "turn on" the fluorescence sensor. Moreover, due to the inhibitory effect of OPs on ALP activity, a dual-mode detection method from fluorescence and colorimetry for OPs was developed. The linear range was from 1 mu g/L to 50 mg/L with a detection limit of 0.4 mu g/L in the fluorescence detection, while the linear range was from 0.1 mu g/L to 50 mg/L with a detection limit of 0.09 mu g/L in colorimetric analysis. Besides, the in-situ imaging of OPs in fresh vegetables was achieved and a visual semi-quantitative detection of OPs on a test paper was realized. Therefore, the fluorescent biosensor may show a potential value in field detection.