CIRCADIAN-RHYTHMS IN MOUSE EPIDERMAL BASAL-CELL PROLIFERATION - VARIATIONS IN COMPARTMENT SIZE, FLUX AND PHASE DURATION
CIRCADIAN-RHYTHMS IN MOUSE EPIDERMAL BASAL-CELL PROLIFERATION - VARIATIONS IN COMPARTMENT SIZE, FLUX AND PHASE DURATION
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DOI：
10.1111/j.1365-2184.1979.tb00154.x
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
ELGJO, K
中科院分区：
文献类型：
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作者：
CLAUSEN, OPF;THORUD, E;ELGJO, K
在无毛[小时/小时奥斯陆株]小鼠表皮基底细胞增殖的几个动力学参数进行了研究，所有参数清楚地表明，在连续2个24小时期间的昼夜节律波动。有丝分裂指数和有丝分裂率进行了研究，在组织切片中，S和G2期DNA含量的细胞的比例进行了测定，通过流式细胞术分离的基底细胞，和[3 H]TdR [胸腺嘧啶脱氧核糖]标记指数和颗粒密度进行了测定，从基底细胞悬浮液涂片放射自显影。根据适应细胞动力学结果的正弦曲线计算每个细胞周期阶段的细胞流入和流出，并确定阶段持续时间。S期细胞的峰值在约2400 h，一组部分同步化的细胞从S期进入G2期，并在清晨穿越G2期和有丝分裂。与从S期流出的变化和通过随后的细胞周期阶段的通量相比，细胞流入S期的波动很小。2400 h前细胞周期穿越S期的延迟可以解释S期细胞的积累以及随后的细胞周期穿越G2期和有丝分裂的部分同步性。清楚地显示了S期、G2期和有丝分裂持续时间的昼夜变化。
Several kinetic parameters of basal cell proliferation in hairless [hr/hr Oslo strain] mouse epidermis were studied and all parameters clearly showed circadian fluctuations during 2 successive 24 h periods. Mitotic indices and the mitotic rate were studied in histological sections; the proportions of cells with S and G2 phase DNA content were measured by flow cytometry of isolated basal cells, and the [3H]TdR [thymine deoxyribose] labeling indices and grain densities were determined by autoradiography in smears from basal cell suspensions. The influx and efflux of cells from each cell cycle phase were calculated from sinusoidal curves adapted to the cell kinetic findings and the phase durations were determined. A peak of cells in S phase was observed at .apprx. 2400 h, and a cohort of partially synchronized cells passed from the S phase to the G2 phase and transversed the G2 phase and mitosis in the early morning. The fluctuations in the influx of cells into the S phase were small compared with the variations in efflux from the S phase and the flux through the subsequent cell cycle phases. The resulting delay in cell cycle traverse through S phase before 2400 h could account for the accumulation of cells in S phase and also the subsequent partial synchrony of cell cycle traverse through the G2 phase and mitosis. Circadian variations in the duration of the S phase, the G2 phase and mitosis were clearly demonstrated.