RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP).
RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP).
复制标题
RFPA，RFPB和RFPC是远红色光摄影（Farlip）的主控制元素。
DOI：
10.3389/fmicb.2015.01303
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发表时间：
2015
影响因子：
5.2
通讯作者：
Bryant DA
中科院分区：
文献类型：
--
作者：
Zhao C;Gan F;Shen G;Bryant DA
陆地蓝藻通常出现在远红光（FRL; λ> 700 nm）强烈富集的小生境中。一些蓝藻表现出复杂和广泛的光适应反应，称为FRL光适应（FaRLiP）。在FaRLiP反应期间，专门的旁系同源蛋白取代三个主要光合复合物的17个核心亚基：光系统（PS）I、PS II和藻胆体。此外，细胞合成叶绿素（Chl）f和Chl d。使用双亲交配从大肠杆菌，我们构建了无效突变体的三个基因，rfpA，rfpB，和rfpC，在蓝藻Chlorogloeopsis fritschii PCC 9212和Chroococcidiopsis thermalis PCC 7203。所得到的突变体不再能够修改它们的光合器官以吸收FRL，不再能够合成Chl f，在白色光下不适当地合成Chl d，并且不能转录FaRLiP基因簇的基因。我们的结论是，RfpA，RfpB和RfpC构成一个FRL激活的信号转导级联，是主控制开关的FaRLiP响应。FRL被认为是激活（或抑制）RfpA的组氨酸激酶活性，从而导致形成RfpB的活性状态，RfpB是关键的反应调节因子和转录激活因子。RfpC可以作为RfpA和RfpB之间的磷酸穿梭。我们的研究结果表明，通过接合反向遗传学将是一个强大的方法，在详细研究的FaRLiP反应。
Terrestrial cyanobacteria often occur in niches that are strongly enriched in far-red light (FRL; λ > 700 nm). Some cyanobacteria exhibit a complex and extensive photoacclimation response, known as FRL photoacclimation (FaRLiP). During the FaRLiP response, specialized paralogous proteins replace 17 core subunits of the three major photosynthetic complexes: Photosystem (PS) I, PS II, and the phycobilisome. Additionally, the cells synthesize both chlorophyll (Chl) f and Chl d. Using biparental mating from Escherichia coli, we constructed null mutants of three genes, rfpA, rfpB, and rfpC, in the cyanobacteria Chlorogloeopsis fritschii PCC 9212 and Chroococcidiopsis thermalis PCC 7203. The resulting mutants were no longer able to modify their photosynthetic apparatus to absorb FRL, were no longer able to synthesize Chl f, inappropriately synthesized Chl d in white light, and were unable to transcribe genes of the FaRLiP gene cluster. We conclude that RfpA, RfpB, and RfpC constitute a FRL-activated signal transduction cascade that is the master control switch for the FaRLiP response. FRL is proposed to activate (or inactivate) the histidine kinase activity of RfpA, which leads to formation of the active state of RfpB, the key response regulator and transcription activator. RfpC may act as a phosphate shuttle between RfpA and RfpB. Our results show that reverse genetics via conjugation will be a powerful approach in detailed studies of the FaRLiP response.