7, 8-Dihydroxyflavone Protects an Endothelial Cell Line from H2O2 Damage.
7, 8-Dihydroxyflavone Protects an Endothelial Cell Line from H2O2 Damage.
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7, 8-二羟基黄酮保护内皮细胞系免受 H2O2 损伤
DOI：
10.1371/journal.pone.0135345
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Qu Z
中科院分区：
文献类型：
--
作者：
Wang B;Zhang Q;Yao R;Liu X;Qu Z
7，8-二羟基黄酮（7，8-dihydroxyflavone，7，8-DHF）是一种选择性TrkB受体激动剂，其神经营养功能已被广泛研究。然而，其在神经组织外的作用却很少被研究。在这项研究中，我们研究了7，8-DHF在EA.hy926细胞中的保护作用，EA.hy926细胞是一种人脐静脉内皮细胞系，暴露于过氧化氢（H2 O2）。我们发现7，8-DHF通过抑制细胞凋亡、减少炎性因子的释放和抑制活性氧的产生，对H2 O2损伤的细胞有明显的保护作用。受体特异性拮抗剂ANA-12可显著阻断7，8-DHF的保护作用，因此7，8-DHF可能通过与TrkB受体结合而发挥其生物学效应。7，8-DHF对EA.hy926细胞的保护作用表明，它将是一个有前途的化合物，将被开发成一个健康的产品，肯定有利于内皮功能和预防心血管疾病。
7, 8-dihydroxyflavone (7, 8-DHF), a selective agonist for TrkB receptors, has been well studied for its neurotrophic functions. However, its roles outside the neural tissues have scarcely been studied as yet. In this study, we investigated the protecting roles of 7, 8-DHF in EA.hy926 cells, a human umbilic vein endothelial cell line which was exposed to hydrogen peroxide (H2O2). We found that 7, 8-DHF significantly protected the cells from being damaged by H2O2 through suppression of apoptosis, attenuation of inflammatory factor releasing and inhibition of reactive oxygen species generation. The potent biological effects of 7, 8-DHF were probably executed via its binding to TrkB receptors because the receptor specific antagonist ANA-12 significantly blocked its protecting effects. The protecting roles of 7, 8-DHF in EA.hy926 cells suggest that it will be a promising compound to be developed into a health product that definitely benefits endothelial functions and prevents cardiovascular diseases.