Serodiagnosis of Helicobacter hepaticus infection in patients with liver and gastrointestinal diseases: western blot analysis and ELISA using a highly specific monoclonal antibody for H. hepaticus antigen
Serodiagnosis of Helicobacter hepaticus infection in patients with liver and gastrointestinal diseases: western blot analysis and ELISA using a highly specific monoclonal antibody for H. hepaticus antigen
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DOI：
10.1007/s00535-011-0414-x
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发表时间：
2011-09-01
影响因子：
6.3
通讯作者：
Fujioka, Toshio
中科院分区：
文献类型：
--
作者：
Murakami, Kazunari;Takahashi, Ryoki;Fujioka, Toshio
背景 肝螺杆菌感染可能与肝脏和胆道疾病有关。为了研究其致病作用，抗 H.方法 采集166例患者的血清样本，其中肝脏疾病69例，上消化道疾病38例，下消化道疾病17例，胆道疾病26例，胰腺疾病16例。 30 份对照血清取自 30 名健康献血者。使用新型单克隆抗体 HR II-51 通过酶联免疫吸附测定 (ELISA) 和蛋白质印迹分析血清样本。与其他疾病组相比，肝病患者 (n = 69) 的肝细胞血清抗体浓度显着升高 (p = 0.014 至
Background Helicobacter hepaticus infection might be associated with liver and biliary tract diseases. To investigate its pathogenic role, the properties of anti-H. hepaticus serum antibody in patients with liver and diseases were elucidated.Methods Serum samples were collected from 166 patients-69 with liver diseases, 38 with upper gastrointestinal diseases, 17 with lower gastrointestinal diseases, 26 with biliary tract diseases, and 16 with pancreas diseases; 30 control sera were obtained from 30 healthy blood donors. Serum samples were analyzed by enzyme-linked immunosorbent assay (ELISA) and western blot using the new monoclonal antibody HR II-51.Results Anti-H. hepaticus serum antibody concentrations in patients with liver disease (n = 69) were significantly increased compared with those in other disease groups (p = 0.014 to