Distribution of tetracycline resistance gene, tet(M), in Gram-positive and Gram-negative bacteria isolated from sediment and seawater at a coastal aquaculture site in Japan
Distribution of tetracycline resistance gene, tet(M), in Gram-positive and Gram-negative bacteria isolated from sediment and seawater at a coastal aquaculture site in Japan
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DOI：
10.1264/jsme2.22.355
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Suzuki, Satoru
中科院分区：
文献类型：
--
作者：
Nonaka, Lisa;Ikeno, Kinuyo;Suzuki, Satoru
我们发现土霉素（OTC）治疗后，在海洋水产养殖场周围的沉积物和海水中的土霉素（OTC）耐药细菌的数量增加。2004年，在日本濑户内海沿岸的一个水产养殖场沿着采集了样品。4月份表层沉积物中对60 μ g mL（-1）土霉素耐药的细菌百分率为6.8%~ 20.0%。在夏季OTC治疗期间，百分比增加，9月份达到53.3%-60.7%。停药后92天，百分比降至22.9%以下。沉积物和海水样品中检测到泰特（M）阳性细菌。泰特（M）在来自不同属的革兰氏阳性和革兰氏阴性细菌中均很明显，并且在类芽孢杆菌属、孢子八叠球菌属、希瓦氏菌属和假交替单胞菌属中是新鉴定的。优势泰特（M）阳性分离株为弧菌属菌株，表明该属是海洋环境中泰特（M）的重要储存库。在5个属的分离物中发现了两种不同的等位基因泰特（M）-A和泰特（M）-B。这些数据表明，水产养殖中使用的药物治疗作为一种选择性压力，促进了耐药细菌数量的增加。
We found increased numbers of oxytetracycline (OTC)-resistant bacteria in sediment and seawater around a marine aquaculture site after OTC therapy. Samples were collected at an aquaculture site along the coast of the Seto Inland Sea, Japan in 2004. In April, the percentage of bacteria resistant to 60 mu g mL(-1) OTC in the surface sediment was 6.8%-20.0%. The percentages increased during OTC therapy in the summer reaching 53.3%-60.7% in September. Ninety-two days after drug cessation, the percentages decreased to below 22.9%. Tet(M)-positive bacteria were detected in the sediment and seawater samples. Tet(M) was evident in both Gram-positive and Gram-negative bacteria from various genera, and was newly identified in Paenibacillus, Sporosarcina, Shewanella, and Pseudoalteromonas. The dominant tet(M)-positive isolates were strains of Vibrio suggesting that this genus is an important reservoir for tet(M) in the marine environment. Two different alleles were found, tet(M)-A and tet(M)-B, each in isolates from five genera. The data suggests drug therapy used in the aquaculture acted as a selective pressure promoting increased numbers of resistant bacteria.