Cross-priming of long lived protective CD8+ T cells against Trypanosoma cruzi infection:: Importance of a TLR9 agonist and CD4+ T cells
Cross-priming of long lived protective CD8+ T cells against Trypanosoma cruzi infection:: Importance of a TLR9 agonist and CD4+ T cells
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DOI：
10.1016/j.vaccine.2007.05.022
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发表时间：
2007-08-10
期刊：
影响因子：
5.5
通讯作者：
Rodrigues, Mauricio M.
中科院分区：
文献类型：
--
作者：
de Alencar, Bruna C. G.;Araujo, Adriano F. S.;Rodrigues, Mauricio M.
我们最近描述了用谷胱甘肽S转移酶融合蛋白（代表无鞭毛体表面蛋白2的氨基酸261-500）对小鼠进行疫苗接种，可以有效地交叉引发保护性CD8（+）T细胞，以抵抗人类原生动物寄生虫克氏锥虫的致命攻击。在这项研究中，我们初步确定这种保护性免疫力是长期存在的。随后，我们研究了TLR9激动剂CpG ODN 1826、TLR4和CD4(+) T细胞对于产生这些保护性CD8(+) T细胞的重要性。我们发现：（i）TLR9激动剂CpG ODN 1826提高了保护性免疫的效率； (ii) TLR4 与特定 CD8(+) T 细胞的启动无关； (iii) CD4(+) T 细胞对于记忆/保护性 CD8(+) T 细胞的启动至关重要。 (c) 2007 Elsevier Ltd. 保留所有权利。
We recently described that vaccination of mice with a gluthatione S transferase fusion protein representing amino acids 261-500 of the Amastigote Surface Protein-2 efficiently cross-primed protective CD8(+) T cells against a lethal challenge with the human protozoan parasite Trypanosoma cruzi. In this study, we initially established that this protective immunity was long lived. Subsequently, we studied the importance of TLR9 agonist CpG ODN 1826, TLR4 and CD4(+) T cells for the generation of these protective CD8(+) T cells. We found that: (i) the TLR9 agonist CpG ODN 1826 improved the efficiency of protective immunity; (ii) TLR4 is not relevant for priming of specific CD8(+) T cells; (iii) CD4(+) T cells are critical for priming of memory/protective CD8(+) T cells. (c) 2007 Elsevier Ltd. All rights reserved.