Integrated Protein Array Screening and High Throughput Validation of 70 Novel Neural Calmodulin-binding Proteins
Integrated Protein Array Screening and High Throughput Validation of 70 Novel Neural Calmodulin-binding Proteins
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DOI：
10.1074/mcp.m900324-mcp200
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发表时间：
2010-06-01
影响因子：
7
通讯作者：
Cahill, Dolores J.
中科院分区：
文献类型：
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作者：
O'Connell, David J.;Bauer, Mikael C.;Cahill, Dolores J.
钙调素是一种重要的调节细胞内过程的响应细胞外刺激介导的钙离子浓度的上升。为了分析人脑中钙调素的蛋白质-蛋白质相互作用，我们在Ca 2+存在下用荧光团标记的钙调素探测了高含量的人蛋白质阵列。该蛋白质阵列包含37，200个冗余蛋白质，包含来自人脑cDNA文库的10，000多个独特的人类神经蛋白质。我们设计了一个筛选，以找到高亲和力（K-D
Calmodulin is an essential regulator of intracellular processes in response to extracellular stimuli mediated by a rise in Ca2+ ion concentration. To profile protein-protein interactions of calmodulin in human brain, we probed a high content human protein array with fluorophore-labeled calmodulin in the presence of Ca2+. This protein array contains 37,200 redundant proteins, incorporating over 10,000 unique human neural proteins from a human brain cDNA library. We designed a screen to find high affinity (K-D