Abundance of ammonia-oxidizing bacteria and archaea in industrial and domestic wastewater treatment systems
Abundance of ammonia-oxidizing bacteria and archaea in industrial and domestic wastewater treatment systems
复制标题
工业和生活废水处理系统中氨氧化细菌和古细菌的丰度
DOI：
10.1111/j.1574-6941.2012.01296.x
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发表时间：
2012-05-01
影响因子：
4.2
通讯作者：
Tang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Bai, Yaohui;Sun, Qinghua;Tang, Xiaoyan
硝化作用在全球氮循环中起着重要作用。氨氧化是硝化的第一步，在废水处理中由氨氧化细菌（AOB）和氨氧化古菌（AOA）共同完成。以往的研究主要集中在自然环境中的分布。在这项研究中，我们定性和定量AOB，AOA，总细菌，总古菌在六个不同的污水处理系统（WTS）使用克隆库和实时PCR技术。结果表明，水质是影响好氧反应器中AOB和AOA分布的重要因素。虽然AOB和AOA都存在于所有样品中，并同时对硝化作用做出贡献，但AOB是三个工业WTS中的优势硝化菌，而AOA在三个家用WTS中占主导地位。这表明AOA可能比AOB对某些有毒化合物更敏感。此外，在工业WTS中的AOB的优势群体是亚硝化单胞菌和亚硝化螺菌; AOA在家庭WTS中的组成非常相似，可能是由于相同的原污水来源。
Nitrification plays a significant role in the global nitrogen cycle. Ammonia oxidation, the first step of nitrification, is performed in wastewater treatment by both ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). Most previous studies focused on their distribution in natural environments. In this study we qualified and quantified AOB, AOA, total bacteria, and total archaea in six different wastewater treatment systems (WTSs) using clone library and real-time PCR techniques. The results revealed that wastewater quality was an essential factor for the distribution of AOB and AOA in aerobic reactors. Although both AOB and AOA were present in all samples and contributed to nitrification simultaneously, AOB were the dominant nitrifiers in the three industrial WTSs, whereas AOA were dominant in the three domestic WTSs. This indicates AOA may be more sensitive to some toxic compounds than AOB. In addition, the dominant groups of AOB in the industrial WTSs were Nitrosomonas and Nitrosospira; the composition of AOA in the domestic WTSs was very similar, possibly due to the same source of raw sewage.