COPPER-INDUCED BINDING OF CELLULAR FACTORS TO YEAST METALLOTHIONEIN UPSTREAM ACTIVATION SEQUENCES
COPPER-INDUCED BINDING OF CELLULAR FACTORS TO YEAST METALLOTHIONEIN UPSTREAM ACTIVATION SEQUENCES
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DOI：
10.1073/pnas.86.1.65
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发表时间：
1989-01-01
影响因子：
11.1
通讯作者：
THIELE, DJ
中科院分区：
文献类型：
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作者：
HUIBREGTSE, JM;ENGELKE, DR;THIELE, DJ
酵母金属硫蛋白基因（CUP 1）的铜诱导转录通过顺式作用上游激活序列（UAS）和反式作用细胞因子发生。我们使用了高分辨率的染色体足迹技术来检测细胞因子与UASCUP 1的相互作用。我们的研究结果表明，一个或多个细胞因子结合UASCUP 1在铜诱导的方式。这种铜诱导的结合在含有阳性调节基因ACE 1的几个拷贝的酵母菌株中增强，并且在含有非功能性（ace 1-Δ 1）基因座的酵母细胞中检测不到。酵母金属硫蛋白基因激活和铜诱导占领UASCUP 1序列之间的相关性表明，金属硫蛋白转录调控因子的顺式作用控制序列的结合是铜诱导的。
Copper-inducible transcription of the yeast metallothionein gene (CUP1) occurs by means of cis-acting upstream activation sequences (UAS) and trans-acting cellular factors. We have used a high-resolution chromosomal foot-printing technique to detect the interaction of cellular factors with UASCUP1. Our results demonstrate that one or more cellular factors bind to UASCUP1 in a copper-inducible fashion. This copper-inducible binding is enhanced in a yeast strain that harbors several copies of the positive regulatory gene ACE1 and is not detectable in yeast cells that contain a nonfunctional (ace1-.DELTA.1) locus. The correlation between yeast metallothionein gene activation and copper-inducible occupation of UASCUP1 sequences suggests that the binding of metallothionein transcriptional regulatory factors to cis-acting control sequences is copper-inducible.