Human experimentation with Neisseria gonorrhoeae:: Progress and goals
Human experimentation with Neisseria gonorrhoeae:: Progress and goals
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DOI：
10.1086/513847
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发表时间：
1999-03-01
影响因子：
6.4
通讯作者：
Cannon, JG
中科院分区：
文献类型：
--
作者：
Cohen, MS;Cannon, JG
感染淋病奈瑟菌会对生殖健康造成不良后果，并促进人类免疫缺陷病毒的传播。淋球菌疫苗发展的一个主要限制是缺乏动物模型。男性志愿者可通过接种尿路疫苗引发尿路感染。数百名患者参与了使用这种实验性感染模型的研究。这些研究有助于确定实验性感染的自然历史，并提供了对淋球菌在体内的表型和基因变异的更好理解。同基因的分子突变可以用来确定淋球菌表面结构的作用，包括菌毛蛋白和转铁蛋白结合蛋白；最近的研究结果表明，淋球菌不能表达转铁蛋白和乳铁蛋白结合蛋白不会导致尿路感染。实验模型已被证明是研究淋球菌感染和专注于疫苗开发的有效手段。此外，这种模式应该允许快速有效地测试疫苗。
Infection with Neisseria gonorrhoeae has adverse consequences for reproductive health and facilitates the transmission of the human immunodeficiency virus. A major limitation in the development of gonococcal vaccines has been the lack of an animal model. Urethral infection can be initiated in male volunteer subjects through urethral inoculation. Several hundred patients have participated in studies using this experimental infection model. These studies have helped define the natural history of experimental infection and provided a better understanding of phenotypic and genotypic variation of gonococci in vivo. Isogenic molecular mutants can be used to define a role for gonococcal surface structures, including pilin and transferrin-binding proteins; recent results demonstrate that gonococci unable to express transferrin- and lactoferrin-binding proteins cannot cause urethral infection. The experimental model has proven to be an efficient means of studying gonococcal infection and focusing vaccine development. In addition, this model should allow vaccines to be tested quickly and efficiently.