|MicroRNA-203 inhibits the proliferation and invasion of U251 glioblastoma cells by directly targeting PLD2 (Retracted Article)
|MicroRNA-203 inhibits the proliferation and invasion of U251 glioblastoma cells by directly targeting PLD2 (Retracted Article)
复制标题
DOI：
10.3892/mmr.2013.1814
复制
发表时间：
2014-02-01
影响因子：
3.4
通讯作者：
Wan, Xin
中科院分区：
文献类型：
--
作者：
Chen, Zigui;Li, Dazhi;Wan, Xin
MicroRNAs(MiRNAs)已被证明在各种类型的癌症的发生和发展中起重要作用。然而，某些抗肿瘤的miRNAs在人类恶性胶质瘤中的确切作用仍有待阐明。本研究旨在揭示microRNA-203(miR-203)在正常脑组织和脑胶质瘤中的表达，并探讨miR-203在人脑胶质母细胞瘤U251细胞增殖和迁移中的作用。实时定量逆转录聚合酶链式反应(RT-PCR)结果显示，miR-203在WHO分级较高的胶质瘤组织中的表达明显低于WHO分级较低的胶质瘤组织和正常脑组织，且随着WHO分级的升高，miR-203的表达呈下降趋势。将miR-203模拟物导入U251细胞后，可显著下调作为miR-203直接靶点的磷脂酶D2(PLD2)的表达。此外，miR-203过表达可显著抑制U251细胞的增殖和侵袭，而PLD2过表达可阻断miR-203模拟物诱导的上述作用。总之，本研究证实了miR-203在胶质瘤中的临床意义，并提示miR-203能够抑制胶质瘤细胞的增殖和侵袭，至少部分是通过抑制PLD2的蛋白表达来实现的。因此，miR-203可能成为开发脑胶质瘤治疗策略的新候选基因。
MicroRNAs (miRNAs) have been demonstrated to be important in the development and progression of various types of cancer. However, the exact roles of certain anti-oncogenic miRNAs in human malignant gliomas remain to be elucidated. The present study aimed to reveal the expression of microRNA-203 (miR-203) in normal brain tissues and gliomas, and to investigate the role of miR-203 in cell proliferation and migration in human glioblastoma U251 cells. Real-time reverse transcription polymerase chain reaction (RT-PCR) showed that the expression of miR-203 in high WHO grade glioma tissues was significantly decreased compared with low WHO grade glioma tissues and normal brain tissues, and its expression demonstrated a decreasing tendency with ascending WHO grades. The transfection of the miR-203 mimic into U251 cells markedly downregulated the expression of phospholipase D2 (PLD2), which was identified as a direct target of miR-203. Furthermore, miR-203 overexpression significantly suppressed the proliferation and invasion of U251 cells, while the overexpression of PLD2 abrogated these effects induced by the miR-203 mimic. In conclusion, the present study demonstrated the clinical significance of miR-203 in gliomas and suggested that miR-203 was able to inhibit the proliferation and invasion of glioma cells, partially at least via suppressing the protein expression of PLD2. Thus, miR-203 may be a novel candidate for the development of therapeutic strategies for gliomas.