Mutation in Torenia fournieri Lind. UFO homolog confers loss of TfLFY interaction and results in a petal to sepal transformation.
Mutation in Torenia fournieri Lind. UFO homolog confers loss of TfLFY interaction and results in a petal to sepal transformation.
复制标题
蓝猪耳的突变。
DOI：
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
N. Ohtsubo
中科院分区：
文献类型：
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作者：
K. Sasaki;H. Yamaguchi;R. Aida;Masahito Shikata;T. Abe;N. Ohtsubo
我们鉴定出一种蓝猪耳（Torenia fournieri Lind.）突变体（编号252），其表现出一种萼片状表型，即第二轮花器官转变为萼片状器官。该突变体没有雄蕊，花器官由萼片和心皮组成。尽管蓝猪耳B类MADS - box基因GLOBOSA（TfGLO）在252突变体中的表达缺失，但未发现TfGLO的突变。在调控拟南芥中B类基因表达的诸如APETALA1（AP1）、LEAFY（LFY）和UNUSUAL FLORAL ORGANS（UFO）等蓝猪耳同源基因中，只有TfUFO转录本的积累在252突变体中减少。此外，在突变体TfUFO的编码区发现了一个错义突变。完整的TfUFO可弥补突变体表型，而突变的TfUFO则不能；此外，TfUFO基因敲低的蓝猪耳转基因表型与突变体表型一致。酵母双杂交分析表明，突变的TfUFO失去了与TfLFY蛋白相互作用的能力。原位杂交分析显示，TfUFO和TfLFY的转录本在同一区域部分积累。这些结果清楚地表明，由于与TfLFY相互作用的丧失，TfUFO的缺陷导致了252突变体的萼片状表型。
We identified a Torenia fournieri Lind. mutant (no. 252) that exhibited a sepaloid phenotype in which the second whorls were changed to sepal-like organs. This mutant had no stamens, and the floral organs consisted of sepals and carpels. Although the expression of a torenia class B MADS-box gene, GLOBOSA (TfGLO), was abolished in the 252 mutant, no mutation of TfGLO was found. Among torenia homologs such as APETALA1 (AP1), LEAFY (LFY), and UNUSUAL FLORAL ORGANS (UFO), which regulate expression of class B genes in Arabidopsis, only accumulation of the TfUFO transcript was diminished in the 252 mutant. Furthermore, a missense mutation was found in the coding region of the mutant TfUFO. Intact TfUFO complemented the mutant phenotype whereas mutated TfUFO did not; in addition, the transgenic phenotype of TfUFO-knockdown torenias coincided with the mutant phenotype. Yeast two-hybrid analysis revealed that the mutated TfUFO lost its ability to interact with TfLFY protein. In situ hybridization analysis indicated that the transcripts of TfUFO and TfLFY were partially accumulated in the same region. These results clearly demonstrate that the defect in TfUFO caused the sepaloid phenotype in the 252 mutant due to the loss of interaction with TfLFY.
影响因子：
11.6
作者：
LEVIN, JZ;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
11.6
作者：
BOWMAN, JL;SMYTH, DR;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM