Differences in metabolite profile between blood plasma and serum
Differences in metabolite profile between blood plasma and serum
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DOI：
10.1016/j.ab.2010.07.015
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发表时间：
2010-11-15
影响因子：
2.9
通讯作者：
Wu, Zimei
中科院分区：
文献类型：
--
作者：
Liu, Linsheng;Aa, Jiye;Wu, Zimei
在代谢组学研究中，血浆和血清被认为具有相似的组成和性质。它们被认为是等同的，这导致研究人员在血清和血浆之间任意选择进行分析。在这里，常规的血清和血浆制备和低分子量化合物的测定，使用气相色谱/飞行时间质谱。采用主成分分析法对采集的数据进行处理，观察到血清和血浆之间代谢产物谱存在显著差异。在72种已鉴定的化合物中，36种（50%）可区分血清和血浆，其中29种和7种代谢物分别在血清和血浆中显示出显著更高的丰度（t检验，P < 0.05）。血液孵育对分析物峰面积有明显影响，血浆的影响比血清更明显，孵育时间较短的影响比孵育时间较长的影响更明显。这些结果突出了选择血清或血浆作为分析样品和规定孵育时间的重要性。由于孵育对血清中分析物峰面积的影响小于血浆中，因此我们建议将血清作为代谢组学研究的首选样本。(C)2010年爱思唯尔公司All rights reserved.
In metabolomic research, blood plasma and serum have been considered to possess similar compositions and properties. Their perceived equivalence has resulted in researchers choosing arbitrarily between serum and plasma for analysis. Here, routine serum and plasma were prepared and their low-molecular-weight compounds were determined using gas chromatography/time-of-flight mass spectrometry. Principal components analysis was applied to process the acquired data, and marked differences in metabolite profiles were observed between serum and plasma. Of the 72 identified compounds, 36 (50%) discriminate serum from plasma, with 29 and 7 metabolites showing a significantly higher abundance (t test, P < 0.05) in serum and plasma, respectively. Incubation of blood had distinct effects on the analyte peak areas, with the effects being more pronounced for plasma than for serum and more pronounced for a shorter incubation than for a longer incubation. These results highlight the importance in choosing serum or plasma as the analytical sample and in stipulating the incubation time. Because incubation affected the analyte peak areas less in serum than in plasma, we recommend serum as the sample of choice in metabolomic studies. (C) 2010 Elsevier Inc. All rights reserved.