Transcriptional regulation of the yeast cytochrome c gene.
Transcriptional regulation of the yeast cytochrome c gene.
复制标题
酵母细胞色素 c 基因的转录调控。
DOI：
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发表时间：
1979
影响因子：
11.1
通讯作者：
B. Hall
中科院分区：
文献类型：
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作者：
R. Zitomer;D. Montgomery;D. L. Nichols;B. Hall
从克隆的酵母iso-1细胞色素c， cycl基因中提取的DNA用于杂交实验，以测量cycl RNA的合成水平和速率。去抑制细胞合成环状RNA的速度是葡萄糖抑制细胞的6倍。将葡萄糖添加到抑制培养物中，cycl基因的转录在2.5分钟内被抑制。在抑制和去抑制条件下以及抑制期间，杂交环状RNA的半衰期为12-13.5 min。结果表明，cycl基因的表达受转录调控。
DNA from the cloned yeast iso-1-cytochrome c, cycl, gene was used in a hybridization assay to measure levels and rates of synthesis of cycl RNA. Derepressed cells synthesized cycl RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cycl gene was repressed within 2.5 min. The half-life of hybridizable cycl RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cycl gene is subject to transcriptional regulation.