A giant virus infecting green algae encodes key fermentation genes
A giant virus infecting green algae encodes key fermentation genes
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感染绿藻的巨型病毒编码关键发酵基因
DOI：
10.1016/j.virol.2018.03.010
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Steward, Grieg F.
中科院分区：
文献类型：
--
作者：
Schvarcz, Christopher R.;Steward, Grieg F.
Mimiviridae家族包含不常见的大型病毒，其中许多是利用自由生活的阿米巴作为宿主分离出来的。虽然这些和其他mimivirids感染海洋异鞭毛和附着原生生物的基因组现已测序，还没有一个mimivirids感染Viridiplantae谱系（绿色藻类和陆地植物）的成员的基因组调查。在这里，我们描述了TetV-1，一种感染世界性绿色藻类Tetraselmis（Chlorodendrophyceae）的mimivirid的668-内切酶完整基因组。分析揭示了以前在病毒中未发现的基因，如甘露醇代谢酶甘露醇1-磷酸脱氢酶，糖降解酶α-半乳糖苷酶，以及关键发酵基因丙酮酸甲酸裂解酶和丙酮酸甲酸裂解酶激活酶。TetV基因组是迄今为止最大的感染光合生物的病毒测序，其基因揭示了病毒操纵宿主代谢的前所未有的机制。
The familyMimiviridaecontains uncommonly large viruses, many of which were isolated using a free-living amoeba as a host. Although the genomes of these and other mimivirids that infect marine heterokont and haptophyte protists have now been sequenced, there has yet to be a genomic investigation of a mimivirid that infects a member of the Viridiplantae lineage (green algae and land plants). Here we characterize the 668-kilobase complete genome of TetV-1, a mimivirid that infects the cosmopolitan green algaTetraselmis(Chlorodendrophyceae). The analysis revealed genes not previously seen in viruses, such as the mannitol metabolism enzyme mannitol 1-phosphate dehydrogenase, the saccharide degradation enzyme alpha-galactosidase, and the key fermentation genes pyruvate formate-lyase and pyruvate formate-lyase activating enzyme. The TetV genome is the largest sequenced to date for a virus that infects a photosynthetic organism, and its genes reveal unprecedented mechanisms by which viruses manipulate their host's metabolism.