Prox1 Facilitates Transfected CHO Cell Proliferation through Activation of the AKT Signaling Pathway
Prox1 Facilitates Transfected CHO Cell Proliferation through Activation of the AKT Signaling Pathway
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Prox1 通过激活 AKT 信号通路促进转染的 CHO 细胞增殖
DOI：
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Shang
中科院分区：
文献类型：
--
作者：
Shang
The homeobox transcription factor Prox1 plays an important role in the development of many embryonic organs. Previous studies indicated that Prox1 facilitates hepatic progenitor-cells proliferation. However, the underlying mechanism of Prox1 in tumor genesis, formation, and progression are poorly understood and need to be exploited. Herein, Chinese Hamster Ovary (CHO) cells were transfected and over-expressed human recombinant Prox1 gene, and developed several stable cell lines of Prox1-CHO after screening. The results indicated that over expression of Prox1 increased CHO cell proliferation in comparison to GFP-CHO and parental CHO cells, and Prox1 increased AKT phosphorylation and up-regulated PI3 Kinase expression. An AKT specific inhibitor-AKTi—X (5 μM) and a PI3 K inhibitor—LY294002 (5 μM) were able to reverse AKT phosphorylation and PI3 K expression induced by Prox1, respectively. Furthermore, AKTi-X but LY-294002 decreased Prox1-CHO cell proliferations at 48 and 72 h. Our results suggest that over expression of Prox1 facilitates CHO cell proliferation via activation of the AKT signaling pathway. This finding provides new insights into the mechanism of Prox1 mediated tumor growth and metastasis where Prox1 is rich.
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影响因子：
3.1
作者：
Shang-zhi Xu
通讯作者：
Shang-zhi Xu
Prospero-DNA 相互作用的结构基础：对发育细胞转录调控的影响。
DOI：
10.1016/j.str.2005.01.023
发表时间：
2005
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Yousef,MohammadS;Matthews,BrianW
通讯作者：
Matthews,BrianW
影响因子：
10.5
作者：
Johnson, Nicole C.;Dillard, Miriam E.;Oliver, Guillermo
通讯作者：
Oliver, Guillermo
影响因子：
3.3
作者：
Shin, JW;Min, M;Hong, YK
通讯作者：
Hong, YK
影响因子：
5.7
作者：
Ryter, JM;Doe, CQ;Matthews, BW
通讯作者：
Matthews, BW