Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin
Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin
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DOI：
10.1016/s0092-8674(02)00757-2
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发表时间：
2002-06-14
期刊：
影响因子：
64.5
通讯作者：
Anderson, DJ
中科院分区：
文献类型：
--
作者：
Mukouyama, Y;Shin, D;Anderson, DJ
神经和血管都是分支结构，但它们的分支模式是独立建立的还是协调建立的尚不清楚。在这里，我们展示了动脉，而不是静脉，在胚胎小鼠肢体皮肤中与周围神经特异性排列。消除周围感觉神经或雪旺细胞的突变阻止了正常的动脉形成，而那些破坏神经的突变则维持了轴突错误的动脉排列。在体外，感觉神经元或雪旺细胞可以诱导离体胚胎内皮细胞中动脉标志物的表达，而VEGF（164/120）介导这种诱导是必要和充分的。这些数据表明，周围神经提供了一个模板，通过局部分泌VEGF来决定皮肤血管分支和动脉分化的器官型模式。
Nerves and blood vessels are branched structures, but whether their branching patterns are established independently or coordinately is not clear. Here we show that arteries, but not veins, are specifically aligned with peripheral nerves in embryonic mouse limb skin. Mutations that eliminate peripheral sensory nerves or Schwann cells prevent proper arteriogenesis, while those that disorganize the nerves maintain the alignment of arteries with misrouted axons. In vitro, sensory neurons or Schwann cells can induce arterial marker expression in isolated embryonic endothelial cells, and VEGF(164/120) is necessary and sufficient to mediate this induction. These data suggest that peripheral nerves provide a template that determines the organotypic pattern of blood vessel branching and arterial differentiation in the skin, via local secretion of VEGF.