Dictyostelium Cells Migrate Similarly on Surfaces of Varying Chemical Composition
Dictyostelium Cells Migrate Similarly on Surfaces of Varying Chemical Composition
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DOI：
10.1371/journal.pone.0087981
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发表时间：
2014-02-06
期刊：
影响因子：
3.7
通讯作者：
Parent, Carole A.
中科院分区：
文献类型：
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作者：
McCann, Colin P.;Rericha, Erin C.;Parent, Carole A.
在细胞迁移期间，伪足锚定需要细胞-基质结合以使细胞向前移动，但与基质的相互作用必须足够弱以允许细胞的部分在典型的突出/缩回循环期间以受控的方式去粘附。哺乳动物细胞主动控制细胞-底物结合，并通过局部含整合素的粘着斑介导机械转导对细胞外条件作出反应。我们问是否机械转导也发生在非整联蛋白介导的迁移过程中，通过检查社会阿米巴Dictyosteelium discoideum，这被认为是结合非特异性表面的运动。我们发现，网骨藻细胞能够调节肌动球蛋白皮质产生的力，以保持最佳的细胞表面接触面积和粘附在各种化学成分的表面上，并且单个细胞在不同表面上以相似的速度和接触面积迁移。相反，在集体迁移期间，如在伤口愈合和转移中观察到的，表面力和膨胀力之间的平衡被改变。我们发现，当细胞接种在不同的表面上时，网骨藻集体迁移动力学受到强烈影响。这些结果表明，细胞-细胞接触的存在下，出现作为Dictyosteoblasts细胞进入发展，改变机制细胞用于迁移表面上的不同组成。
During cell migration, cell-substrate binding is required for pseudopod anchoring to move the cell forward, yet the interactions with the substrate must be sufficiently weak to allow parts of the cell to de-adhere in a controlled manner during typical protrusion/retraction cycles. Mammalian cells actively control cell-substrate binding and respond to extracellular conditions with localized integrin-containing focal adhesions mediating mechanotransduction. We asked whether mechanotransduction also occurs during non-integrin mediated migration by examining the motion of the social amoeba Dictyostelium discoideum, which is thought to bind non-specifically to surfaces. We discovered that Dictyostelium cells are able to regulate forces generated by the actomyosin cortex to maintain optimal cell-surface contact area and adhesion on surfaces of various chemical composition and that individual cells migrate with similar speed and contact area on the different surfaces. In contrast, during collective migration, as observed in wound healing and metastasis, the balance between surface forces and protrusive forces is altered. We found that Dictyostelium collective migration dynamics are strongly affected when cells are plated on different surfaces. These results suggest that the presence of cell-cell contacts, which appear as Dictyostelium cells enter development, alter the mechanism cells use to migrate on surfaces of varying composition.