Antigenic variation in the influenza A virus nonstructural protein, NS1.
Antigenic variation in the influenza A virus nonstructural protein, NS1.
复制标题
甲型流感病毒非结构蛋白 NS1 的抗原变异。
DOI：
10.1016/0042-6822(83)90123-x
复制
发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Webster,RG
中科院分区：
文献类型：
--
作者：
Brown,LE;Hinshaw,VS;Webster,RG
使用针对从感染细胞的细胞质分离的纯化的NS 1内含物制备的单克隆抗体，检查由A型流感病毒编码的非结构（NS 1）蛋白的抗原结构。竞争性放射免疫分析表明，A/WSN/33（H1N1）NS 1上存在3个不同的重叠抗原区。使用感染的细胞裂解物的免疫沉淀研究表明，A/WSN/33 NS 1上的抗原决定簇对于由人、猪、马和禽类来源的广泛病毒编码的NS 1蛋白是共同的。然而，发现几种禽类菌株编码抗原性变体NS 1蛋白，其在一个或多个抗原区域中具有广泛变化或在提示抗原漂移的区域内的表位中具有小变化。表面抗原亚型与NS 1蛋白的抗原谱之间没有相关性。本研究中显示的NS 1蛋白的抗原关系与可用的序列数据一致。
The antigenic structure of the nonstructural (NS1) protein encoded by influenza type A virus was examined using monoclonal antibodies prepared against purified NSl inclusions isolated from the cytoplasm of infected cells. Topographical analysis by competitive radioimmunoassay indicated that three different overlapping antigenic regions were present on the NS1 of A/WSN/33 (HlNl). Immunoprecipitation studies using infected cell lysates showed that antigenic determinants on A/WSN/33 NSl are common to NSl proteins encoded by a wide range of viruses of human, swine, equine, and avian origin. Several avian strains, however, were found to encode antigenically variant NSl proteins which had either extensive changes in one or more antigenic regions or small changes in epitopes within a region suggestive of antigenic drift. There was no correlation between surface antigen subtype and the antigenic profile of the NSl protein. The antigenic relationships of NSl proteins shown in this study are in agreement with the available sequence data.