The effect of 17O on the relaxation of an amide proton within a hydrogen bond.
The effect of 17O on the relaxation of an amide proton within a hydrogen bond.
复制标题
17O 对氢键内酰胺质子弛豫的影响。
DOI：
10.1007/bf00182279
复制
发表时间：
1995
影响因子：
2.7
通讯作者：
Redfield,AG
中科院分区：
文献类型：
--
作者：
Halkides,CJ;Redfield,AG
在有和无17 O标记的情况下，测定了rasp 21·GDP中Gly 13的酰胺氢的多量子相干弛豫速率。标记和未标记样品之间未观察到显著差异，尽管基于其化学位移，Gly 13的酰胺基团与β-磷酸的氢键比典型的短。对于大分子，其中氧原子是氢键的受体，偶极耦合之间17 O和氢是不太可能被观察到，除了极短的H-键。
The relaxation rates of the multiple-quantum coherence for the amide hydrogen of Gly13inrasp21·GDP were determined in the presence and absence of17O labeling in the β-phosphate of GDP. No significant difference could be observed between labeled and unlabeled samples, in spite of the fact that the hydrogen bond from the amide group of Gly13to the β-phosphate is shorter than is typical, based on its chemical shift. For macromolecules in which an oxygen atom is the acceptor of a hydrogen bond, dipolar coupling between17O and hydrogen is unlikely to be observable, except for extremely short H-bonds.