Sustained growth of Ichthyophthirius multifiliis at low temperature in the laboratory.
Sustained growth of Ichthyophthirius multifiliis at low temperature in the laboratory.
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DOI：
10.2307/3284065
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发表时间：
1995-12
期刊：
影响因子：
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通讯作者：
Jane G. Noe;Harry W. Dickerson
中科院分区：
文献类型：
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作者：
Jane G. Noe;Harry W. Dickerson
应用和基础研究的纤毛虫Ichthyophthirius multifiliis，专性寄生的淡水鱼，需要通过鱼类宿主，以保持实验室的股票。然而，连续重复传代导致寄生虫克隆随时间衰老。由于生长和发育与水温直接相关，我们的目标是在低温下培养寄生虫，以延长生物体在鱼身上的停留时间，从而减少：（1）传代次数和（2）随着时间的推移维持寄生虫所需的鱼的数量。水温从25 ℃降低到9 ℃导致癍点叉尾鮰的生长显著减慢（寄生虫在9 ℃下在鱼身上保留20.4天，而在25 ℃下为5-6天），在活力、抗原性或感染性方面没有明显的变化。低温生长被认为是一种稳定维持I。在实验室里进行多丝培养。
Applied and basic research on the ciliate Ichthyophthirius multifiliis, an obligate parasite of freshwater fishes, requires passage on fish hosts to maintain laboratory stocks. However, continual repeated passage results in senescence of parasite clones over time. Because growth and development are directly correlated to water temperature, our objective was to grow the parasite at low temperature in order to extend the period that the organism remains on the fish, thus reducing: (1) the number of passages and (2) the number of fish required to maintain the parasite over time. Lowering of water temperature from 25 C to 9 C resulted in significant slowing of growth on channel catfish (parasites remained on fish for 20.4 days at 9 C, as compared to 5-6 days at 25 C), with no discernible changes in viability, antigenicity, or infectivity. Low-temperature growth is proposed as an improved method for stable maintenance of I. multifiliis cultures in the laboratory.