Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore
Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore
复制标题
使用铱 (III) 发光体对线粒体动力学进行超分辨率跟踪
DOI：
10.1002/smll.201802166
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发表时间：
2018-10-11
期刊：
影响因子：
13.3
通讯作者：
Diao, Jiajie
中科院分区：
文献类型：
--
作者：
Chen, Qixin;Jin, Chengzhi;Diao, Jiajie
将发光过渡金属配合物与超分辨率显微镜相结合是一种长期观察活细胞亚细胞结构动态的极好策略。然而，目前还不清楚铱（III）配合物是否适用于一种特殊类型的超分辨率技术，结构照明显微镜（SIM），图像亚细胞结构。在此，描述了在SIM下跟踪活细胞中的线粒体动力学的铱（III）染料。该染料显示出优异的特异性和光稳定性以及令人满意的细胞渗透性。当使用SIM对线粒体成像时，实现了约80 nm的分辨率，允许清晰地观察线粒体嵴的结构。该染料用于监测和定量相对于溶酶体的线粒体动力学，包括线粒体自噬中涉及的融合，以及不同条件下新发现的线粒体-溶酶体接触（MLC）。当p62、NDP 52、OPTN、NBR 1和TAX 1BP 1五种受体被敲除时，MLC保持完整，融合消失，这表明这两个过程是独立的。
Combining luminescent transition metal complex with super-resolution microscopy is an excellent strategy for the long-term visualization of the dynamics of subcellular structures in living cells. However, it remains unclear whether iridium(III) complexes are applicable for a particular type of super-resolution technique, structured illumination microscopy (SIM), to image subcellular structures. Herein, an iridium(III) dye, to track mitochondrial dynamics in living cells under SIM is described. The dye demonstrates excellent specificity and photostability and satisfactory cell permeability. While using SIM to image mitochondria, an approximate to 80 nm resolution is achieved that allows the clear observation of the structure of mitochondrial cristae. The dye is used to monitor and quantify mitochondrial dynamics relative to lysosomes, including fusion involved in mitophagy, and newly discovered mitochondria-lysosome contact (MLC) under different conditions. The MLC remains intact and fusion vanishes when five receptors, p62, NDP52, OPTN, NBR1, and TAX1BP1, are knocked out, suggesting that these two processes are independent.