INSULIN AND IGF-1 RECEPTORS IN A HUMAN INTESTINAL ADENOCARCINOMA CELL-LINE (CACO-2) - REGULATION OF NA+ GLUCOSE-TRANSPORT ACROSS THE BRUSH-BORDER
INSULIN AND IGF-1 RECEPTORS IN A HUMAN INTESTINAL ADENOCARCINOMA CELL-LINE (CACO-2) - REGULATION OF NA+ GLUCOSE-TRANSPORT ACROSS THE BRUSH-BORDER
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DOI：
10.3109/10799899309063266
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
BEAN, TL
中科院分区：
文献类型：
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作者：
MACDONALD, RS;THORNTON, WH;BEAN, TL
胰岛素和IGF-1受体都存在于肠粘膜细胞中，尽管它们在该组织中的作用尚不清楚。我们在人腺癌细胞系Caco-2中对这些受体进行了表征，并研究了它们在这些细胞中调节葡萄糖运输和吸收的作用。Caco-2细胞表现出特异性胰岛素和IGF-1受体。它们还结合了细胞松弛素B，表明存在葡萄糖转运蛋白样蛋白。当生长在膜上时，Caco-2细胞形成柱状，双极细胞紧密连接。该单层膜选择性地转运了d -葡萄糖和甲基- d -葡萄糖，完全排除了l-葡萄糖、d -甘露醇和菊糖。葡萄糖的吸收是通过Na+/葡萄糖共转运体发生的，这可以从向顶膜添加葡萄糖后短路电流的变化中看出。当在几种条件下检查时，胰岛素和IGF-1都没有影响葡萄糖在Caco-2单层上的运输，也没有影响细胞产生乳酸。提示Caco-2细胞的胰岛素和IGF-1受体不调节葡萄糖转运。
Both insulin and IGF-1 receptors are present in intestinal mucosal cells, although their role in this tissue is unclear. We have characterized these receptors in a human adenocarcinoma cell line, Caco-2, and examined their role in the regulation of glucose transport and absorption in these cells. The Caco-2 cells demonstrated specific insulin and IGF-1 receptors. They also bound cytochalasin B, suggesting the presence of a glucose transporter-like protein. When grown on membranes, the Caco-2 cells formed columnar, bipolar cells with tight junctions. The monolayer selectively transported D-glucose and methyl-D-glucose, with complete exclusion of L-glucose, D-mannitol and inulin. The absorption of glucose across the monolayer occurred via a Na+/glucose cotransporter, as indicated by a change in short circuit current after addition of glucose to the apical membrane. When examined under several conditions, neither insulin nor IGF-1 had an affect on the transport of glucose across the Caco-2 monolayer, nor the production of lactate by the cells. It is concluded that the insulin and IGF-1 receptors of Caco-2 cells do not regulate glucose transport.