In vitro and In vivo Optical Imaging Using Water-Dispersible, Noncytotoxic, Luminescent, Silica-Coated Quantum Rods
In vitro and In vivo Optical Imaging Using Water-Dispersible, Noncytotoxic, Luminescent, Silica-Coated Quantum Rods
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DOI：
10.1021/cm902610f
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发表时间：
2010-04-13
影响因子：
8.6
通讯作者：
Prasad, Paras N.
中科院分区：
文献类型：
--
作者：
Kumar, Rajiv;Ding, Hong;Prasad, Paras N.
报道了一种制备单分散、生物相容、二氧化硅包覆的CdSe/CdS/ZnS量子棒（QRs）的简便方法，并将其用作活体和体外光学生物成像的探针。二氧化硅包覆过程包括将前体正硅酸乙酯（TEOS）在封装在Igepal CO-520/环己烷微乳液的反胶束核心内的QR上缩聚。已通过TEM、DLS和光致发光光谱表征了二氧化硅涂覆的QR。二氧化硅壳的厚度也被证明是通过改变TEOS和QR的比例来调节的。用二氧化硅包被的QR处理的不同细胞系（RAW 1和RAW）的共聚焦生物成像已经显示出纳米颗粒的稳健摄取，而不需要特异性靶向分子。为了评价纳米颗粒的细胞毒性，已经进行了48小时的MTS测定。在直接瘤内注射纳米颗粒后，皮下异种移植荷瘤小鼠的体内成像显示在注射10分钟内来自肿瘤的荧光。这项研究表明，这些二氧化硅涂层的QR可以潜在地用于体外和体内的长期靶向成像研究。
A facile method for synthesis of monodisperse, biocompatible, silica coated CdSe/CdS/ZnS quantum rods (QRs) and their use as probes for in vitro and in vivo optical bioimaging is presented. The silica coating procedure involves condensation-polymerization of the precursor tetraethylorthosilicate (TEOS) over QRs encapsulated within the reverse micellar core of Igepal CO-520/cyclohexane microemulsion. The silica-coated QRs have been characterized by TEM, DLS, and photoluminescence spectroscopy. The silica shell thickness has also been shown to be tuned by varying the ratio of TEOS and QRs. Confocal bioimaging of different cell lines (Pane 1 and RAW), treated with silica coated QRs, has shown a robust uptake of the nanoparticles, without the need of specific targeting molecules. To evaluate the cytotoxicity of the nanoparticles, MTS assay has been carried out over a period of 48 h. In vivo imaging of subcutaneously xenografted tumor-bearing mice, following direct intratumoral injection of the nanoparticles, have shown fluorescence from the tumor within 10 min of injection. This study suggests that these silica-coated QRs can be potentially used for long-term targeted imaging studies in vitro and in vivo.